Use of Phosphorus-32 in Microassay for Nucleic Acid Synthesis in Escherichia coli
- 14 August 1959
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 130 (3372), 386-387
- https://doi.org/10.1126/science.130.3372.386
Abstract
A method was developed to facilitate studies of ribonucleic acid and deoxyribonucleic acid synthesis in growing bacterial cultures of small volume. The nucleic acid fractions were separated by means of a modified Schmidt-Thannhauser procedure applied to micro quantities of P32-labeled cells collected on membrane filters. The validity and usefulness of the method are discussed.Keywords
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