Mucosal and Peripheral Lin − HLA-DR + CD11c/123 − CD13 + CD14 − Mononuclear Cells Are Preferentially Infected during Acute Simian Immunodeficiency Virus Infection
- 15 January 2012
- journal article
- Published by American Society for Microbiology in Journal of Virology
- Vol. 86 (2), 1069-1078
- https://doi.org/10.1128/jvi.06372-11
Abstract
Massive infection of memory CD4 T cells is a hallmark of early simian immunodeficiency virus (SIV) infection, with viral infection peaking at day 10 postinfection (p.i.), when a majority of memory CD4 T cells in mucosal and peripheral tissues are infected. It is not clear if mononuclear cells from the monocyte and macrophage lineages are similarly infected during this early phase of explosive HIV and SIV infections. Here we show that, at day 10 p.i., Lin − HLA-DR + CD11c/123 − CD13 + CD14 − macrophages in the jejunal mucosa were infected, albeit at lower levels than CD4 memory T cells. Interestingly, Lin − HLA-DR + CD11c/123 − CD13 + CD14 − macrophages in peripheral blood, like their mucosal counterparts, were preferentially infected compared to Lin − HLA-DR + CD11c/123 − CD13 + CD14 + monocytes, suggesting that differentiated macrophages were selectively infected by SIV. CD13 + CD14 − macrophages expressed low levels of CD4 compared to CD4 T cells but expressed similar levels of CCR5 as lymphocytes. Interestingly, CD13 + CD14 − macrophages expressed Apobec3G at lower levels than CD13 + CD14 + monocytes, suggesting that intracellular restriction may contribute to the differential infection of mononuclear subsets. Taken together, our results suggest that CD13 + CD14 − macrophages in mucosal and peripheral tissues are preferentially infected very early during the course of SIV infection.Keywords
This publication has 60 references indexed in Scilit:
- Innate and adaptive immune correlates of vaccine and adjuvant-induced control of mucosal transmission of SIV in macaquesProceedings of the National Academy of Sciences of the United States of America, 2010
- Use of a Modified α-N-Acetylgalactosaminidase in the Development of Enzyme Replacement Therapy for Fabry DiseaseAmerican Journal of Human Genetics, 2009
- The level of monocyte turnover predicts disease progression in the macaque model of AIDSBlood, 2009
- α4+β7hiCD4+ memory T cells harbor most Th-17 cells and are preferentially infected during acute SIV infectionMucosal Immunology, 2009
- Gag- and Nef-specific CD4+T cells recognize and inhibit SIV replication in infected macrophages early after infectionProceedings of the National Academy of Sciences of the United States of America, 2009
- HIV and SIV infection: the role of cellular restriction and immune responses in viral replication and pathogenesisAPMIS, 2009
- Analyzing real-time PCR data by the comparative CT methodNature Protocols, 2008
- Human macrophages support persistent transcription from unintegrated HIV-1 DNAVirology, 2007
- Mutational Alteration of Human Immunodeficiency Virus Type 1 Vif Allows for Functional Interaction with Nonhuman Primate APOBEC3GJournal of Virology, 2006
- Quantification of latent tissue reservoirs and total body viral load in HIV-1 infectionNature, 1997