Markers of glycemic control in the mouse: comparisons of 6-h- and overnight-fasted blood glucoses to Hb A1c
- 1 October 2008
- journal article
- research article
- Published by American Physiological Society in American Journal of Physiology-Endocrinology and Metabolism
- Vol. 295 (4), E981-E986
- https://doi.org/10.1152/ajpendo.90283.2008
Abstract
The present studies examined the relationship between fasting blood glucose and Hb A1cin C57BL/6J, DBA/2J, and KK/HlJ mice with and without diabetes mellitus. Daily averaged blood glucose levels based on continuous glucose monitoring and effects of 6-h vs. overnight fasting on blood glucose were determined. Daily averaged blood glucose levels were highly correlated with Hb A1c, as determined with a hand-held automated device using an immunodetection method. R2values were 0.90, 0.95, and 0.99 in KK/HIJ, C57BL/6J, and DBA/2J, respectively. Six-hour fasting blood glucose correlated more closely with the level of daily averaged blood glucose and with Hb A1cthan did blood glucose following an overnight fast. To validate the immunoassay-determined Hb A1c, we also measured total glycosylated hemoglobin using boronate HPLC. Hb A1cvalues correlated well with total glycosylated hemoglobin in all three strains but were relatively lower than total glycosylated hemoglobin in diabetic DBA/2J mice. These results show that 6-h fasting glucose provides a superior index of glycemic control and correlates more closely with Hb A1cthan overnight-fasted blood glucose in these strains of mice.Keywords
This publication has 40 references indexed in Scilit:
- Deletion of Nicotinamide Nucleotide TranshydrogenaseDiabetes, 2006
- Nephron Number and Renal Risk in Hypertension and DiabetesJournal of the American Society of Nephrology, 2005
- Red blood cell survival in chronic renal failureAmerican Journal of Kidney Diseases, 2004
- Biological Variation in HbA1c Predicts Risk of Retinopathy and Nephropathy in Type 1 DiabetesDiabetes Care, 2004
- How valid is fasting plasma glucose as a parameter of glycemic control in non-insulin-using patients with type 2 diabetes?Diabetes Care, 1999
- Intensive blood-glucose control with sulphonylureas or insulin compared with conventional treatment and risk of complications in patients with type 2 diabetes (UKPDS 33)The Lancet, 1998
- Optimizing Blood Glucose Control in Type 2 Diabetes: an Approach Based on Fasting Blood Glucose MeasurementsDiabetic Medicine, 1988
- THE BASAL PLASMA GLUCOSE: A SIMPLE RELEVANT INDEX OF MATURITY-ONSET DIABETESClinical Endocrinology, 1981
- Hemoglobin Beta Chain Structural Variation in Mice: Evolutionary and Functional ImplicationsScience, 1972
- Inherited Electrophoretic Hemoglobin Patterns among 20 Inbred Strains of MiceScience, 1958