Cloning and expression of human endothelin‐1 receptor cDNA
Open Access
- 5 August 1991
- journal article
- Published by Wiley in FEBS Letters
- Vol. 287 (1-2), 23-26
- https://doi.org/10.1016/0014-5793(91)80007-p
Abstract
We isolated a human endothelin‐1 (ET‐1) receptor cDNA from a human placenta cDNA library. The cDNA encodes a 427‐amino acid protein with seven putative transmembrane domains. The rank order of the binding to the receptor expressed in COS‐7 cells was: ET‐1 ⩾ ET‐2 ⪢ ET‐3. The receptor expressed in Xenopus oocytes showed a potent electrophysiological response to 1 × 10−7 M ET‐1 under voltage clamp at −60 mV, while a much weaker response was produced by 1 × 10−7 M ET‐3. Northern blot analysis with RNA from human tissues revealed a single band with a size of 4.3 kb in a wide variety of human tissues, especially highly in the blood vessel.Keywords
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