Generation of a glutathione peroxidase-like mimic using bioimprinting and chemical mutation

Abstract

Molecular imprinting of albumin with N,S-bis(2,4-dinitrophenyl)glutathione (GSH-2DNP), a glutathione (GSH) derivative, leads to an imprinted protein with GSH binding sites; chemical mutation of the protein results in formation of an artificial enzyme with high glutathione peroxidase (GPX) activity.