Microarray comparative genomic hybridization detection of chromosomal imbalances in uterine cervix carcinoma
Open Access
- 9 July 2005
- journal article
- Published by Springer Science and Business Media LLC in BMC Cancer
- Vol. 5 (1), 77
- https://doi.org/10.1186/1471-2407-5-77
Abstract
Background: Chromosomal Comparative Genomic Hybridization (CGH) has been applied to all stages of cervical carcinoma progression, defining a specific pattern of chromosomal imbalances in this tumor. However, given its limited spatial resolution, chromosomal CGH has offered only general information regarding the possible genetic targets of DNA copy number changes. Methods: In order to further define specific DNA copy number changes in cervical cancer, we analyzed 20 cervical samples (3 pre-malignant lesions, 10 invasive tumors, and 7 cell lines), using the GenoSensor microarray CGH system to define particular genetic targets that suffer copy number changes. Results: The most common DNA gains detected by array CGH in the invasive samples were located at the RBP1-RBP2 (3q21-q22) genes, the sub-telomeric clone C84C11/T3 (5ptel), D5S23 (5p15.2) and the DAB2 gene (5p13) in 58.8% of the samples. The most common losses were found at the FHIT gene (3p14.2) in 47% of the samples, followed by deletions at D8S504 (8p23.3), CTDP1-SHGC- 145820 (18qtel), KIT (4q11-q12), D1S427-FAF1 (1p32.3), D9S325 (9qtel), EIF4E (eukaryotic translation initiation factor 4E, 4q24), RB1 (13q14), and DXS7132 (Xq12) present in 5/17 (29.4%) of the samples. Conclusion: Our results confirm the presence of a specific pattern of chromosomal imbalances in cervical carcinoma and define specific targets that are suffering DNA copy number changes in this neoplasm.Keywords
This publication has 41 references indexed in Scilit:
- Genetic analysis identifies putative tumor suppressor sites at 2q35–q36.1 and 2q36.3–q37.1 involved in cervical cancer progressionOncogene, 2003
- Common fragile sites are preferential targets for HPV16 integrations in cervical tumorsOncogene, 2003
- Comprehensive molecular cytogenetic characterization of cervical cancer cell linesGenes, Chromosomes and Cancer, 2003
- Epigenetic Regulation of a Novel Tumor Suppressor Gene (hDAB2IP) in Prostate Cancer Cell LinesOnline Journal of Public Health Informatics, 2003
- Molecular events associated with dysplastic morphologic transformation and initiation of ovarian tumorigenicityCancer, 2002
- Expression of the p53 Homologue p63 in Early Cervical NeoplasiaGynecologic Oncology, 2001
- Comparative genomic hybridization: Uses and limitationsSeminars in Hematology, 2000
- Restoration of positioning control following Disabled-2 expression in ovarian and breast tumor cellsOncogene, 2000
- Genome-wide analysis of DNA copy number variation in breast cancer using DNA microarraysNature Genetics, 1999
- Gain of chromosome 3q defines the transition from severe dysplasia to invasive carcinoma of the uterine cervix.Proceedings of the National Academy of Sciences of the United States of America, 1996