Fluorescence Relaxation Dynamics of Acridine Orange in Nanosized Micellar Systems and DNA
- 30 March 2007
- journal article
- Published by American Chemical Society (ACS) in The Journal of Physical Chemistry B
- Vol. 111 (16), 4189-4199
- https://doi.org/10.1021/jp067156r
Abstract
In this paper, we report a detailed study of the fluorescence relaxation dynamics of a well-known fluorescent DNA intercalator, acridine orange (AO), in reverse micelles (RM), micelles, and DNA using picosecond resolved fluorescence spectroscopy. Solvation studies of AO in AOT reverse micelles (RM) containing water indicate the locations of AO close to the interface and those in RM containing NaOH; there are two types of AOone in the nonpolar oil phase and the other at the interface. The bound water at the reverse micellar interface is found to be much more rigid than that at the micellar interface of sodium dodecyl sulfate (SDS) micelles. Dynamic light scattering (DLS) studies allow for the determination of the hydrodynamic radius and the overall tumbling motion of the macromolecules. Wobbling-in-cone data analysis of the temporal fluorescence anisotropy decay allows for determination of restriction on the motion of fluorophores attached to the macromolecules. This model further applied to AO-intercalated genomic DNA and synthetic oligonucleotides within their structural integrity (as confirmed through circular dichroism (CD) studies) shows that AO experiences less restriction in genomic salmon sperm DNA compared with that in synthetic oligonucleotides, and among the oligonucleotides, the ones with AT base pairs are much more rigid. This study would invoke further research on the dynamical nature of AO in restricted environments.Keywords
This publication has 64 references indexed in Scilit:
- Photophysical Processes in the Complexes of DNA with Ethidium Bromide and Acridine Orange: A Femtosecond StudyThe Journal of Physical Chemistry B, 2000
- Water in oil colloïdal droplets used as microreactorsAdvances in Colloid and Interface Science, 1993
- Dielectric properties of anionic and nonionic surfactant microemulsionsLangmuir, 1990
- Reverse micelles as hosts for proteins and small moleculesBiochimica et Biophysica Acta (BBA) - Reviews on Biomembranes, 1988
- Anisotropic motions in intercalative DNA-dye complexesThe Journal of Physical Chemistry, 1986
- Picosecond fluorescence anisotropy decay in the ethidium/DNA complexThe Journal of Physical Chemistry, 1983
- Torsion and bending of nucleic acids studied by subnanosecond time-resolved fluorescence depolarization of intercalated dyesThe Journal of Chemical Physics, 1982
- Singlet energy transfer between nucleic acid bases and dyes in intercalation complexesThe Journal of Physical Chemistry, 1980
- Theory of twisting and bending of chain macromolecules; analysis of the fluorescence depolarization of DNAThe Journal of Chemical Physics, 1979
- A Nuclear Magnetic Resonance Investigation of the Aggregation of Acridine Orange in Aqueous SolutionJournal of the American Chemical Society, 1967