The Culture of Skin a Review of Theories and Experimental Methods
- 1 July 1976
- journal article
- review article
- Published by Elsevier BV in Journal of Investigative Dermatology
- Vol. 67 (1), 58-65
- https://doi.org/10.1111/1523-1747.ep12512483
Abstract
Two main criticisms can be leveled against the standard methods of skin culture: they are poorly quantifiable and the cultured cell populations are heterogeneous. A new technique based mainly on enzymatic dissociation allows specific cell types to be extracted from the skin before cultivation. In this way, separate cultures of epidermal keratinocytes and dermal fibroblasts can be obtained from the same piece of skin. These purified systems have been used to study the kinetics of epidermal cell growth and to quantify the effect of various chemically defined substances on the growth and differentiation of keratinocytes. With further refinements in technique, purified populations of melanocytes can be extracted. The co-culture of pigmented melanocytes with albino keratinocytes has been proposed as a model to study pigment donation in vitro. The usual organ culture technique, including the use of large explants of skin immersed in the culture fluid, has been modified to show that adult human skin partially regenerates in vitro and that mitotic activity goes on for months in the regenerated epidermis. The use of nucleic acid hybridization techniques, combined with skin cell cultures from human tumors, opens new avenues of research on human cancerKeywords
This publication has 61 references indexed in Scilit:
- Growth Stimulation of Cultured Postembryonic Epidermal Cells by Vitamin a Acid**From the Department of Dermatology, University of Munich, 8 München 2, GermanyJournal of Investigative Dermatology, 1974
- Cyclic AMP-Elevating Agents Inhibit Proliferation of Keratinizing Guinea Pig Epidermal CellsDifferentiation, 1974
- Keratinization in dispersed cell cultures of adult guinea-pig ear skinBritish Journal of Dermatology, 1974
- Studies of Proliferative Capabilities in Isolated Epidermal Basal and Differentiated CellsJournal of Investigative Dermatology, 1971
- A fine-structure study of the human epidermal melanosome complex and its acid phosphatase activityJournal of Ultrastructure Research, 1968
- Structure of viruses of the papilloma-polyoma type IV. Analysis of tilting experiments in the electron microscopeJournal of Molecular Biology, 1968
- ELECTRON MICROSCOPIC IDENTIFICATION OF EPITHELIAL CELLS IN TISSUE CULTURE OF ORAL MUCOUS MEMBRANEThe Journal of cell biology, 1967
- Culture of Adult Human Skin:in vitro Growth and Keratinization of Epidermal CellsNature, 1966
- Pigment Donation In VitroJournal of Investigative Dermatology, 1964
- The Cultivation of Cells from Adult Epidermis**From the M.R.C. Unit for Research on the Experimental Pathology of the Skin, University of Birmingham, England.Journal of Investigative Dermatology, 1960