Cultivation of cell–polymer tissue constructs in simulated microgravity
- 20 May 1995
- journal article
- Published by Wiley in Biotechnology & Bioengineering
- Vol. 46 (4), 306-313
- https://doi.org/10.1002/bit.260460403
Abstract
Tissue‐engineered cartilage was cultivated under conditions of simulated microgravity using rotating bioreactors. Rotation randomized the effects of gravity on inoculated cells (chondrocytes) and permitted their attachment to three‐dimensional (3D) synthetic, biodegradable polymer scaffolds that were freely suspended within the vessel. After 1 week of cultivation, the cells regenerated a cartilaginous extracellular matrix (ECM) consisting of glycosaminoglycan (GAG) and collagen types I and II. Tissue constructs grown in simulated microgravity had higher GAG contents and thinner outer capsules than control constructs grown in turbulent spinner flasks. Two fluid dynamic regimes of simulated microgravity were identified, depending on the vessel rotation speed: (i) a settling regime in which the constructs were maintained in a state of continuous free‐fall close to a stationary point within the vessel and (ii) an orbiting regime in which the constructs orbited around the vessel spin axis. In the settling regime, the numerically calculated relative fluid‐construct velocity was comparable to the experimentally measured construct settling velocity (2–3 cm/s). A simple mathematical model was used in conjunction with measured construct physical properties to determine the hydrodynamic drag force and to estimate the hydrodynamic stress at the construct surface (1.5 dyn/cm2). Rotating bioreactors thus provide a powerful research tool for cultivating tissue‐engineered cartilage and studying 3D tissue morphogenesis under well‐defined fluid dynamic conditions. © 1995 John Wiley & Sons, Inc.Keywords
This publication has 20 references indexed in Scilit:
- Joint resurfacing using allograft chondrocytes and synthetic biodegradable polymer scaffoldsJournal of Biomedical Materials Research, 1994
- Kinetics of chondrocyte growth in cell‐polymer implantsBiotechnology & Bioengineering, 1994
- Cultivation of cell‐polymer cartilage implants in bioreactorsJournal of Cellular Biochemistry, 1993
- Three-dimensional growth and differentiation of ovarian tumor cell line in high aspect rotating-wall vessel: Morphologic and embryologic considerationsJournal of Cellular Biochemistry, 1993
- Neocartilage formation in vitro and in vivo using cells cultured on synthetic biodegradable polymersJournal of Biomedical Materials Research, 1993
- Cartilage and diarthrodial joints as paradigms for hierarchical materials and structuresBiomaterials, 1992
- Biosynthetic response of cartilage explants to dynamic compressionJournal of Orthopaedic Research, 1989
- Improved quantitation and discrimination of sulphated glycosaminoglycans by use of dimethylmethylene blueBiochimica et Biophysica Acta (BBA) - General Subjects, 1986
- Theoretical studies on living systems in the absence of mechanical stressJournal of Theoretical Biology, 1965
- The laws of bone architectureJournal of Anatomy, 1917