Full-length genome sequences of five hepatitis C virus isolates representing subtypes 3g, 3h, 3i and 3k, and a unique genotype 3 variant
- 1 March 2013
- journal article
- Published by Microbiology Society in Journal of General Virology
- Vol. 94 (3), 543-548
- https://doi.org/10.1099/vir.0.049668-0
Abstract
We characterized the full-length genomes of five distinct hepatitis C virus (HCV)-3 isolates. These represent the first complete genomes for subtypes 3g and 3h, the second such genomes for 3k and 3i, and of one novel variant presently not assigned to a subtype. Each genome was determined from 18–25 overlapping fragments. They had lengths of 9579–9660 nt and each contained a single ORF encoding 3020–3025 aa. They were isolated from five patients residing in Canada; four were of Asian origin and one was of Somali origin. Phylogenetic analysis using 64 partial NS5B sequences differentiated 10 assigned subtypes, 3a–3i and 3k, and two additional lineages within genotype 3. From the data of this study, HCV-3 full-length sequences are now available for six of the assigned subtypes and one unassigned. Our findings should add insights to HCV evolutionary studies and clinical applications.This publication has 35 references indexed in Scilit:
- Complete genomic sequences for hepatitis C virus subtypes 4b, 4c, 4d, 4g, 4k, 4l, 4m, 4n, 4o, 4p, 4q, 4r and 4tJournal of General Virology, 2009
- The unique HCV genotype distribution and the discovery of a novel subtype 6u among IDUs co‐infected with HIV‐1 in Yunnan, ChinaJournal of Medical Virology, 2008
- Use of Sequence Analysis of the NS5B Region for Routine Genotyping of Hepatitis C Virus with Reference to C/E1 and 5′ Untranslated Region SequencesJournal of Clinical Microbiology, 2007
- Hepatitis C virus complete genome sequences identified from China representing subtypes 6k and 6n and a novel, as yet unassigned subtype within genotype 6Journal of General Virology, 2006
- Hepatitis C Virus Subtyping by a Core-Envelope 1-Based Reverse Transcriptase PCR Assay with Sequencing and Its Use in Determining Subtype Distribution among Danish PatientsJournal of Clinical Microbiology, 2003
- Sequence analysis of hepatitis C virus genotypes 1 to 5 reveals multiple novel subtypes in the Benelux countriesJournal of General Virology, 1995
- Molecular epidemiology of hepatitis C virus infection among intravenous drug usersJournal of Hepatology, 1995
- Prevalence of hepatitis C virus sequence variants in south-east AsiaJournal of General Virology, 1995
- Analysis of hepatitis C virus genotypes by a line probe assay and correlation with antibody profilesJournal of Hepatology, 1994
- Hepatitis B virus subtypes and hepatitis C virus genotypes in patients with chronic liver disease in NepalHepatology, 1994