Serum Metabolite Profiling of Human Colorectal Cancer Using GC−TOFMS and UPLC−QTOFMS
- 4 September 2009
- journal article
- research article
- Published by American Chemical Society (ACS) in Journal of Proteome Research
- Vol. 8 (10), 4844-4850
- https://doi.org/10.1021/pr9004162
Abstract
Colorectal carcinogenesis involves the overexpression of many immediate-early response genes associated with growth and inflammation, which significantly alters downstream protein synthesis and small-molecule metabolite production. We have performed a serum metabolic analysis to test the hypothesis that the distinct metabolite profiles of malignant tumors are reflected in biofluids. In this study, we have analyzed the serum metabolites from 64 colorectal cancer (CRC) patients and 65 healthy controls using gas chromatography time-of-flight mass spectrometry (GC−TOFMS) and Acquity ultraperformance liquid chromatography-quadrupole time-of-flight mass spectrometry (Acquity UPLC−QTOFMS). Orthogonal partial least-squares discriminate analysis (OPLS-DA) models generated from GC−TOFMS and UPLC−QTOFMS metabolic profile data showed robust discrimination from CRC patients and healthy controls. A total of 33 differential metabolites were identified using these two analytical platforms, five of which were detected in both instruments. These metabolites potentially reveal perturbation of glycolysis, arginine and proline metabolism, fatty acid metabolism and oleamide metabolism, associated with CRC morbidity. These results suggest that serum metabolic profiling has great potential in detecting CRC and helping to understand its underlying mechanisms.Keywords
This publication has 33 references indexed in Scilit:
- Metabolomic profiles delineate potential role for sarcosine in prostate cancer progressionNature, 2009
- Cancer Statistics, 2008CA: A Cancer Journal for Clinicians, 2008
- Metabolite profiling of human colon carcinoma – deregulation of TCA cycle and amino acid turnoverMolecular Cancer, 2008
- l-Arginine Reduces Cell Proliferation and Ornithine Decarboxylase Activity in Patients with Colorectal Adenoma and AdenocarcinomaClinical Cancer Research, 2007
- Evaluation of metabolite profiles as biomarkers for the pharmacological effects of thiazolidinediones in Type 2 diabetes mellitus patients and healthy volunteersBritish Journal of Clinical Pharmacology, 2007
- Application of ethyl chloroformate derivatization for gas chromatography–mass spectrometry based metabonomic profilingAnalytica Chimica Acta, 2007
- Metabolic profiling using combined GC–MS and LC–MS provides a systems understanding of aristolochic acid‐induced nephrotoxicity in ratFEBS Letters, 2007
- Cannabinoids and cancer: potential for colorectal cancer therapyBiochemical Society Transactions, 2005
- Colon cancerCritical Reviews in Oncology/Hematology, 2004
- 'Metabonomics': understanding the metabolic responses of living systems to pathophysiological stimuli via multivariate statistical analysis of biological NMR spectroscopic dataXenobiotica, 1999