Longitudinal shift in diabetic wound microbiota correlates with prolonged skin defense response
Open Access
- 28 July 2010
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences of the United States of America
- Vol. 107 (33), 14799-14804
- https://doi.org/10.1073/pnas.1004204107
Abstract
Diabetics frequently suffer from chronic, nonhealing wounds. Although bacterial colonization and/or infection are generally acknowledged to negatively impact wound healing, the precise relationship between the microbial community and impaired wound healing remains unclear. Because the host cutaneous defense response is proposed to play a key role in modulating microbial colonization, we longitudinally examined the diabetic wound microbiome in tandem with host tissue gene expression. By sequencing 16S ribosomal RNA genes, we show that a longitudinal selective shift in wound microbiota coincides with impaired healing in diabetic mice (Leprdb/db; db/db). We demonstrate a parallel shift in longitudinal gene expression that occurs in a cluster of genes related to the immune response. Further, we establish a correlation between relative abundance of Staphylococcus spp. and the expression of cutaneous defense response genes. Our data demonstrate that integrating two types of global datasets lends a better understanding to the dynamics governing host–microbe interactions.Keywords
This publication has 33 references indexed in Scilit:
- Commensal bacteria regulate Toll-like receptor 3–dependent inflammation after skin injuryNature Medicine, 2009
- The NIH Human Microbiome ProjectGenome Research, 2009
- Topographical and Temporal Diversity of the Human Skin MicrobiomeScience, 2009
- Treatment of Staphylococcus aureus Colonization in Atopic Dermatitis Decreases Disease SeverityPEDIATRICS, 2009
- Defects in Innate Immunity Predispose C57BL/6J-Leprdb/LeprdbMice to Infection byStaphylococcus aureusInfection and Immunity, 2009
- A diversity profile of the human skin microbiotaGenome Research, 2008
- Bacteriology of Moderate-to-Severe Diabetic Foot Infections and In Vitro Activity of Antimicrobial AgentsJournal of Clinical Microbiology, 2007
- The ribosomal database project (RDP-II): introducing myRDP space and quality controlled public dataNucleic Acids Research, 2006
- Who are we?EMBO Reports, 2006
- Gene set enrichment analysis: A knowledge-based approach for interpreting genome-wide expression profilesProceedings of the National Academy of Sciences of the United States of America, 2005