Human cytotoxic T-cell recognition of a synthetic peptide of myelin basic protein
- 1 September 1989
- journal article
- research article
- Published by Wiley in Annals of Neurology
- Vol. 26 (3), 342-346
- https://doi.org/10.1002/ana.410260306
Abstract
Previous studies with a panel of myelin basic protein (MBP)–specific human T‐cell clones suggested a clustering of epitopes in the middle and at the C terminus of the molecule. The current study demonstrates that 19 of 40 clones recognize a synthetic peptide corresponding to residues 152 to 170 of the human MBP molecule and that 9 clones recognize a synthetic peptide corresponding to residues 86 to 105. Myelin basic protein–specific cytotoxic activity was restricted to the clones that recognized peptide 152–170, and this peptide served as a preferential cytotoxic T‐cell target when attached to an autologous B‐cell line. The specificity of MBP‐directed cytotoxic activity appears to be much more restricted than the specificity demonstrated for proliferative activity.Keywords
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