Expression of platelet‐endothelial cell adhesion molecule‐1 (PECAM‐1) during melanoma‐induced angiogenesis in vivo
- 1 October 1993
- journal article
- Published by Wiley in Journal of Cutaneous Pathology
- Vol. 20 (5), 399-406
- https://doi.org/10.1111/j.1600-0560.1993.tb00661.x
Abstract
Recent ultrastructural data indicate that tumor-induced angiogenesis involves polarized outgrowth of endothelial cells from established vessels into tumor parenchyma and interstitium. Efforts to define the cytoarchitecture of the angiogenic response and to ascertain molecular determinants of polarized endothelial migration have been impeded by lack of sensitive and specific immunologic markers for endothelium and vessel wall components. In this study, we utilized monoclonal antibody to platelet-endothelial cell adhesion molecule-1 (PECAM-1), a cell-cell adhesion molecule belonging to the immunoglobulin superfamily, to determine extent and patterns of angiogenesis in metastatic melanomas before (N = 7) and after (N = 3) induction of tumor-infiltrating lymphocyte responses provoked by administration of experimental melanoma vaccine. PECAM-1 proved to be a more reliable marker for angiogenesis than antibodies to von Willebrand Factor. Although both normal and tumor vessels exhibited prominent staining for PECAM-1, different patterns of endothelial reactivity were observed. Formation of new vessels was associated with i) PECAM-1 redistribution from a constitutive circumferential membrane pattern to a pattern restricted to cell-cell junctions; ii) formation of endothelial cords formed by cell-cell interactions; and iii) eventual development of open lumens. Vessels within inflamed (vaccine-treated) and non-inflamed melanomas did not differ with regard to patterns of PECAM-1 expression. Forming vessels of inflamed melanomas failed to express the cytokine-inducible activation marker, E-selectin. Although normal microvessels and reactive vessels of healing wounds demonstrated prominent staining for alpha 6 laminin receptor, vessels within melanomas showed apparently diminished expression.(ABSTRACT TRUNCATED AT 250 WORDS)Keywords
This publication has 16 references indexed in Scilit:
- Autologous Melanoma Vaccine Induces Inflammatory Responses in Melanoma Metastases: Relevance to Immunologic Regression and Immunothererapy.Journal of Investigative Dermatology, 1993
- TNFα Induces E-Selectin Expression and PECAM-1 (CD31) Redistribution in Extracutaneous TissuesEndothelium, 1993
- Aberrant integrin expression during epidermal wound healing and in psoriatic epidermis.JCI Insight, 1992
- Heterogeneity of vascular endothelial cells with relevance to diagnosis of vascular tumours.Journal of Clinical Pathology, 1992
- Molecular and cellular properties of PECAM-1 (endoCAM/CD31): a novel vascular cell-cell adhesion molecule.The Journal of cell biology, 1991
- EndoCAM: a novel endothelial cell-cell adhesion molecule.The Journal of cell biology, 1990
- PECAM-1 (CD31) Cloning and Relation to Adhesion Molecules of the Immunoglobulin Gene SuperfamilyScience, 1990
- Intercellular Adhesion Molecule Expression in the Evolving Human Cutaneous Delayed Hypersensitivity ReactionJournal of Investigative Dermatology, 1989
- A human endothelial cell-restricted, externally disposed plasmalemmal protein enriched in intercellular junctions.The Journal of Experimental Medicine, 1989
- Induction and detection of a human endothelial activation antigen in vivo.The Journal of Experimental Medicine, 1986