Improved determination of plasmid copy number using quantitative real-time PCR for monitoring fermentation processes
Open Access
- 1 January 2008
- journal article
- Published by Springer Science and Business Media LLC in Microbial Cell Factories
- Vol. 7 (1), 6
- https://doi.org/10.1186/1475-2859-7-6
Abstract
Recombinant protein production in Escherichia coli cells is a complex process, where among other parameters, plasmid copy number, structural and segregational stability of plasmid have an important impact on the success of productivity. It was recognised that a method for accurate and rapid quantification of plasmid copy number is necessary for optimization and better understanding of this process. Lately, qPCR is becoming the method of choice for this purpose. In the presented work, an improved qPCR method adopted for PCN determination in various fermentation processes was developed.Keywords
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