LC‐MS Data Processing with MAVEN: A Metabolomic Analysis and Visualization Engine
Open Access
- 1 March 2012
- journal article
- unit
- Published by Wiley in Current Protocols in Bioinformatics
- Vol. 37 (1), 14.11.1-14.11.23
- https://doi.org/10.1002/0471250953.bi1411s37
Abstract
MAVEN is an open‐source software program for interactive processing of LC‐MS‐based metabolomics data. MAVEN enables rapid and reliable metabolite quantitation from multiple reaction monitoring data or high‐resolution full‐scan mass spectrometry data. It automatically detects and reports peak intensities for isotope‐labeled metabolites. Menu‐driven, click‐based navigation allows visualization of raw and analyzed data. Here we provide a User Guide for MAVEN. Step‐by‐step instructions are provided for data import, peak alignment across samples, identification of metabolites that differ strongly between biological conditions, quantitation and visualization of isotope‐labeling patterns, and export of tables of metabolite‐specific peak intensities. Together, these instructions describe a workflow that allows efficient processing of raw LC‐MS data into a form ready for biological analysis. Curr. Protoc. Bioinform. 37:14.11.1‐14.11.23. © 2012 by John Wiley & Sons, Inc.This publication has 29 references indexed in Scilit:
- Riboneogenesis in YeastCell, 2011
- Metabolomic Analysis and Visualization Engine for LC−MS DataAnalytical Chemistry, 2010
- Metabolomic Analysis via Reversed-Phase Ion-Pairing Liquid Chromatography Coupled to a Stand Alone Orbitrap Mass SpectrometerAnalytical Chemistry, 2010
- A guided tour of the Trans‐Proteomic PipelineProteomics, 2010
- Metabolic systems biologyFEBS Letters, 2009
- Metabolomics‐driven quantitative analysis of ammonia assimilation in E. coliMolecular Systems Biology, 2009
- Analytical strategies for LC–MS-based targeted metabolomicsJournal of Chromatography B, 2008
- XCMS2: Processing Tandem Mass Spectrometry Data for Metabolite Identification and Structural CharacterizationAnalytical Chemistry, 2008
- Metabolic flux analysis in a nonstationary system: Fed-batch fermentation of a high yielding strain of E. coli producing 1,3-propanediolMetabolic Engineering, 2007
- XCMS: Processing Mass Spectrometry Data for Metabolite Profiling Using Nonlinear Peak Alignment, Matching, and IdentificationAnalytical Chemistry, 2006