Determination of Thiamphenicol in Serum and Cerebrospinal Fluid with High-Pressure Liquid Chromatography
- 1 January 1981
- journal article
- research article
- Published by Informa UK Limited in Journal of Liquid Chromatography
- Vol. 4 (1), 145-154
- https://doi.org/10.1080/01483918108064804
Abstract
A procedure for quantitation of thiamphenicol in serum and CSF was developed using high-pressure liquid chromatography. The drug was extracted from biological samples with methanol and separated by reverse-phase high-pressure liquid chromatography. Detection and subsequent quantitation were performed at 254 nm by on-line UV spectrophotometry. After the i.m. administration of a single dose of 1 g of thiamphenicol to a patient, a poor transmission of the drug across the hematoencephalic barrier was demonstrated by this assay.This publication has 10 references indexed in Scilit:
- Determination of serum chloramphenicol by high-performance liquid chromatographyJournal of Chromatography B: Biomedical Sciences and Applications, 1979
- High-Pressure Liquid Chromatographic Assay of Chloramphenicol in Biological FluidsAntimicrobial Agents and Chemotherapy, 1979
- Determination of chloramphenicol in serum and cerebrospinal fluid with high-pressure liquid chromatographyJournal of Antimicrobial Chemotherapy, 1978
- A rapid gas chromatographic determination of thiamphenicol in plasma and amniotic fluidJournal of Chromatography A, 1976
- Gas chromatographic determination and gas chromatographic-mass spectrometric analysis of chloramphenicol, thiamphenicol and their metabolitesJournal of Chromatography A, 1975
- Structure-activity relation of chloramphenicolsJournal of Medicinal Chemistry, 1973
- Determination of thiamphenicol in biological fluids by gas chromatographyJournal of Chromatography A, 1969
- An Apparent Correlation between the in Vitro Activity of Chloramphenicol Analogs and Electronic PolarizabilityJournal of Medicinal Chemistry, 1967
- Quantification and Prediction of the Biological Activities of Chloramphenicol Analogs by Microbial Kinetics1Journal of Medicinal Chemistry, 1966
- Absorption and Urinary Excretion of Chloramphenicol and 2 Analogues, Thiocymetin and U-15,442 in Normal Men.Experimental Biology and Medicine, 1960