Biofunctionalization, cytotoxicity, and cell uptake of lanthanide doped hydrophobically ligated NaYF4 upconversion nanophosphors
- 1 November 2008
- journal article
- research article
- Published by AIP Publishing in Journal of Applied Physics
- Vol. 104 (9), 094308
- https://doi.org/10.1063/1.3008028
Abstract
Surface biofunctionalization of the hydrophobic lanthanide ion doped hexagonal phase upconversion nanophosphors (UCNPs) was achieved by introducing amino and carboxyl groups, respectively. Amino groups were added by using the 3-aminopropyltrimethoxysilane reaction after a thin layer of coating. The carboxyl groups on surface were added directly by coating modified amphiphilic polyacrylic acid polymer. Experimental studies of cytotoxicity and cell uptake of UCNPs were conducted. The cytotoxicity analysis of the functionalized UCNPs was conducted by methylthiazol tetrazolium assays. Cell uptake was accomplished by incubating the UCNPs with human osteosarcoma cells and proved by transmission electron microscopy. The results showed that the functionalized UCNPs had very low toxicity compared with the control group, while UCNPs taken into the cells indicated that they had very high biocompatibility. The imaging of UCNPs, which were incubated with AB12 mouse mesothelioma cells and excited by 1 W 980 nm light, showed individual particles with visible light emission. These results exhibited promising applications of functionalized UCNPs in cell imaging and photodynamic therapy.
Keywords
This publication has 27 references indexed in Scilit:
- Water-Soluble NaYF4:Yb,Er(Tm)/NaYF4/Polymer Core/Shell/Shell Nanoparticles with Significant Enhancement of Upconversion FluorescenceChemistry of Materials, 2006
- Monodisperse Silica‐Coated Polyvinylpyrrolidone/NaYF4 Nanocrystals with Multicolor Upconversion Fluorescence EmissionAngewandte Chemie-International Edition, 2006
- Synthesis of Hexagonal‐Phase NaYF4:Yb,Er and NaYF4:Yb,Tm Nanocrystals with Efficient Up‐Conversion FluorescenceAdvanced Functional Materials, 2006
- High-Quality Sodium Rare-Earth Fluoride Nanocrystals: Controlled Synthesis and Optical PropertiesJournal of the American Chemical Society, 2006
- Upconversion spectroscopy and properties of NaYF4 doped with , and/orJournal of Luminescence, 2006
- In Vivo and Scanning Electron Microscopy Imaging of Upconverting Nanophosphors inCaenorhabditiselegansNano Letters, 2005
- Highly efficient near-infrared to visible up-conversion process in NaYF4:Er3+,Yb3+Journal of Luminescence, 2005
- Infrared up-converting phosphors for bioassaysIEE Proceedings - Nanobiotechnology, 2005
- Hexagonal Sodium Yttrium Fluoride Based Green and Blue Emitting Upconversion PhosphorsChemistry of Materials, 2004
- Up-converting phosphor reporters for nucleic acid microarraysNature Biotechnology, 2001