Deciphering the Factors for Nodulation and Symbiosis of Mesorhizobium Associated with Cicer arietinum in Northwest India
Open Access
- 16 December 2019
- journal article
- research article
- Published by MDPI AG in Sustainability
- Vol. 11 (24), 7216
- https://doi.org/10.3390/su11247216
Abstract
The compatibility between rhizobia and legumes for nitrogen-fixing nodules and the stages of root hair curling, formation of infection thread, and nodulation initiation have been vitally studied, but the factors for the sustainable root surface colonization and efficient symbiosis within chickpea and rhizobia have been poorly investigated. Hence, we aimed to analyze phenotypic properties and phylogenetic relationships of root-nodule bacteria associated with chickpea (Cicer arietinum) in the north-west Indo Gangetic Plains (NW-IGP) region of Uttar Pradesh, India. In this study, 54 isolates were recovered from five agricultural locations. Strains exhibited high exopolysaccharide production and were capable of survival at 15–42 °C. Assays for phosphate solubilization, catalase, oxidase, Indole acetic acid (IAA) production, and 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase activity revealed that all the tested isolates possessed plant growth-promoting potential. Metabolic profiling using Biolog plates indicated that patterns of substrate utilization differed considerably among isolates. A biofilm formation assay showed that isolates displayed a nearly four-fold range in their capacity for biofilm development. Inoculation experiments indicated that all isolates formed nodules on chickpea, but they exhibited more than a two-fold range in symbiotic efficiency. No nodules were observed on four other legumes (Phaseolus vulgaris, Pisum sativum, Lens culinaris, and Vigna mungo). Concatenated sequences from six loci (gap, edD, glnD, gnD, rpoB, and nodC) supported the assignment of all isolates to the species Mesorhizobium ciceri, with strain M. ciceri Ca181 as their closest relative.Funding Information
- Science and Engineering Research Board (PDF/2015/000801)
This publication has 66 references indexed in Scilit:
- Phenotyping Chickpeas and Pigeonpeas for Adaptation to DroughtFrontiers in Physiology, 2012
- An integrated view of biofilm formation in rhizobiaFEMS Microbiology Letters, 2010
- Signals, Regulatory Networks, and Materials That Build and Break Bacterial BiofilmsMicrobiology and Molecular Biology Reviews, 2009
- DnaSP v5: a software for comprehensive analysis of DNA polymorphism dataBioinformatics, 2009
- High Catalase Production by Rhizobium radiobacter Strain 2-1Journal of Bioscience and Bioengineering, 2008
- Chromosomal and Symbiotic Relationships of Rhizobia Nodulating Medicago truncatula and M. laciniataApplied and Environmental Microbiology, 2007
- Diversity and geographical distribution of indigenous soybean-nodulating bradyrhizobia in JapanSoil Science and Plant Nutrition, 2006
- The Ecology and Biogeography of Microorganisms on Plant SurfacesAnnual Review of Phytopathology, 2000
- Signal Transduction in Rhizobium-Induced Nodule FormationPlant Physiology, 1996
- Multiple Range and Multiple F TestsBiometrics, 1955