High prevalence of extended-spectrum- -lactamase-producing Enterobacteriaceae in organic and conventional retail chicken meat, Germany
Open Access
- 6 August 2012
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of Antimicrobial Chemotherapy
- Vol. 67 (11), 2631-2634
- https://doi.org/10.1093/jac/dks295
Abstract
To determine the prevalence of extended-spectrum β-lactamase (ESBL) production in Enterobacteriaceae in retail chicken meat in Germany. A total of 399 chicken meat samples from nine supermarket chains, four organic food stores and one butcher's shop in two geographically distinct regions (Berlin and Greifswald) were screened for ESBL production using selective agar. Phenotypic ESBL isolates were tested for blaTEM, blaCTX-M and blaSHV genes using PCR and DNA sequencing. Antibiotic coresistances were determined and strain typing was performed using PCR-based phylogenetic grouping and XbaI-PFGE. A total of 185 confirmed ESBL isolates were obtained from 175 samples (43.9%) from all tested sources. The majority of isolates were Escherichia coli producing ESBL types SHV-12 (n = 82), CTX-M-1 (n = 77) and TEM-52 (n = 16). No differences could be observed in the prevalence of ESBL producers between organic and conventional samples. 73.0% of the ESBL producers showed coresistance to tetracycline, 35.7% to co-trimoxazole and 7.6% to ciprofloxacin. Strain typing of selected E. coli isolates from Berlin revealed identical macrorestriction patterns for several isolates from samples taken from the same stores. This is the first comprehensive study from Germany showing a high prevalence of TEM-, CTX-M- and SHV-type ESBLs in Enterobacteriaceae isolated from retail chicken meat. The high rate of coresistance to different classes of antibiotics in the ESBL producers might reflect the common veterinary usage of these and related substances. There is an urgent need to further evaluate the role of poultry in the transmission of highly resistant ESBL-producing bacteria in humans.Keywords
This publication has 11 references indexed in Scilit:
- Comparison of ESBL contamination in organic and conventional retail chicken meatInternational Journal of Food Microbiology, 2012
- Extended-Spectrum B-Lactamase Genes of Escherichia coli in Chicken Meat and Humans, the NetherlandsEmerging Infectious Diseases, 2011
- Dutch patients, retail chicken meat and poultry share the same ESBL genes, plasmids and strainsClinical Microbiology & Infection, 2011
- Comparison of the sales of veterinary antibacterial agents between 10 European countriesJournal of Antimicrobial Chemotherapy, 2010
- Salmonella entericaSerovar Typhi with CTX-M β-Lactamase, GermanyEmerging Infectious Diseases, 2009
- Emergence of carbapenem-non-susceptible extended-spectrum β-lactamase-producing Klebsiella pneumoniae isolates at the university hospital of Tübingen, GermanyJournal of Medical Microbiology, 2009
- Prevalence and spread of extended-spectrum β-lactamase-producing Enterobacteriaceae in EuropeClinical Microbiology & Infection, 2008
- Spread of Escherichia coli Strains with High-Level Cefotaxime and Ceftazidime Resistance between the Community, Long-Term Care Facilities, and Hospital InstitutionsJournal of Clinical Microbiology, 2006
- High Levels of Antimicrobial Coresistance among Extended-Spectrum-β-Lactamase-Producing EnterobacteriaceaeAntimicrobial Agents and Chemotherapy, 2005
- Rapid and Simple Determination of the Escherichia coli Phylogenetic GroupApplied and Environmental Microbiology, 2000