Granulocyte/macrophage colony-stimulating factor and interleukin 3 release from human peripheral blood eosinophils and neutrophils.
Open Access
- 1 September 1991
- journal article
- Published by Rockefeller University Press in The Journal of Experimental Medicine
- Vol. 174 (3), 745-748
- https://doi.org/10.1084/jem.174.3.745
Abstract
Human peripheral blood eosinophils released eosinophil survival-enhancing activity when stimulated with the calcium ionophore, ionomycin. The release of activity was detected as early as 3 h after stimulation and was inhibited by an immunomodulating agent, cyclosporin A. The survival-enhancing activity was completely abolished by treatment with anti-interleukin 3 (IL-3) and anti-granulocyte/macrophage colony-stimulating factor (GM-CSF) monoclonal antibodies. Moreover, IL-3 and GM-CSF were measurable in ionomycin-stimulated eosinophil supernatants by immunoassay. Eosinophils produced approximately one-half as much IL-3 and one-fifth as much GM-CSF as ionomycin-stimulated mononuclear cells. Neutrophils also produced IL-3 and GM-CSF, but the amounts were less than those produced by eosinophils. These observations suggest a novel role for eosinophils in pathophysiology of allergic inflammation and host defense mechanisms.This publication has 13 references indexed in Scilit:
- Human eosinophils express transforming growth factor alpha.The Journal of Experimental Medicine, 1990
- Regulation of parasite-induced eosinophilia: selectively increased interleukin 5 production in helminth-infected patients.The Journal of Experimental Medicine, 1990
- Murine eosinophils and IL-1: alpha IL-1 mRNA detection by in situ hybridization. Production and release of IL-1 from peritoneal eosinophils.The Journal of Immunology, 1990
- T Lymphocyte-Derived Colony-Stimulating FactorsAdvances in Immunology, 1990
- Hemopoietins for Eosinophils: Glycoprotein Hormones that Regulate the Development of Inflammation in Eosinophilia-Associated DiseaseHematology/Oncology Clinics of North America, 1989
- Production of the haemopoietic growth factors GM-CSF and interleukin-3 by mast cells in response to IgE receptor-mediated activationNature, 1989
- IgA-induced eosinophil degranulation.1989
- Purified colony-stimulating factors enhance the survival of human neutrophils and eosinophils in vitro: a rapid and sensitive microassay for colony-stimulating factors.1986
- Cyclosporin A inhibits T-cell growth factor gene expression at the level of mRNA transcription.Proceedings of the National Academy of Sciences of the United States of America, 1984
- Distinctive cationic proteins of the human eosinophil granule: major basic protein, eosinophil cationic protein, and eosinophil-derived neurotoxin.The Journal of Immunology, 1983