Phospholipase A2 Is Required for PIN-FORMED Protein Trafficking to the Plasma Membrane in theArabidopsisRoot
Open Access
- 1 June 2010
- journal article
- Published by Oxford University Press (OUP) in Plant Cell
- Vol. 22 (6), 1812-1825
- https://doi.org/10.1105/tpc.110.074211
Abstract
Phospholipase A2 (PLA2), which hydrolyzes a fatty acyl chain of membrane phospholipids, has been implicated in several biological processes in plants. However, its role in intracellular trafficking in plants has yet to be studied. Here, using pharmacological and genetic approaches, the root hair bioassay system, and PIN-FORMED (PIN) auxin efflux transporters as molecular markers, we demonstrate that plant PLA2s are required for PIN protein trafficking to the plasma membrane (PM) in the Arabidopsis thaliana root. PLA2α, a PLA2 isoform, colocalized with the Golgi marker. Impairments of PLA2 function by PLA2α mutation, PLA2-RNA interference (RNAi), or PLA2 inhibitor treatments significantly disrupted the PM localization of PINs, causing internal PIN compartments to form. Conversely, supplementation with lysophosphatidylethanolamine (the PLA2 hydrolytic product) restored the PM localization of PINs in the pla2α mutant and the ONO-RS-082–treated seedling. Suppression of PLA2 activity by the inhibitor promoted accumulation of trans-Golgi network vesicles. Root hair–specific PIN overexpression (PINox) lines grew very short root hairs, most likely due to reduced auxin levels in root hair cells, but PLA2 inhibitor treatments, PLA2α mutation, or PLA2-RNAi restored the root hair growth of PINox lines by disrupting the PM localization of PINs, thus reducing auxin efflux. These results suggest that PLA2, likely acting in Golgi-related compartments, modulates the trafficking of PIN proteins.Keywords
This publication has 56 references indexed in Scilit:
- Auxin: A Trigger for Change in Plant DevelopmentCell, 2009
- Phospholipase A2β mediates light-induced stomatal opening in ArabidopsisJournal of Experimental Botany, 2008
- Endosidin1 defines a compartment involved in endocytosis of the brassinosteroid receptor BRI1 and the auxin transporters PIN2 and AUX1Proceedings of the National Academy of Sciences of the United States of America, 2008
- Arabidopsis PLDζ2Regulates Vesicle Trafficking and Is Required for Auxin ResponsePlant Cell, 2007
- Functional Conservation of a Root Hair Cell-Specificcis-Element in Angiosperms with Different Root Hair Distribution PatternsPlant Cell, 2006
- Subcellular Trafficking of the Arabidopsis Auxin Influx Carrier AUX1 Uses a Novel Pathway Distinct from PIN1Plant Cell, 2006
- Dynamic Response of Prevacuolar Compartments to Brefeldin A in Plant CellsPlant Physiology, 2006
- Manipulation of Arabidopsis fatty acid amide hydrolase expression modifies plant growth and sensitivity to N -acylethanolaminesProceedings of the National Academy of Sciences of the United States of America, 2006
- PINOID Positively Regulates Auxin Efflux inArabidopsisRoot Hair Cells and Tobacco CellsPlant Cell, 2006
- Auxin inhibits endocytosis and promotes its own efflux from cellsNature, 2005