Mono‐ and binuclear Zn‐β‐lactamase from Bacteroides fragilis: catalytic and structural roles of the zinc ions
Open Access
- 30 October 1998
- journal article
- Published by Wiley in FEBS Letters
- Vol. 438 (1-2), 137-140
- https://doi.org/10.1016/s0014-5793(98)01289-7
Abstract
The Bacteroides fragilis Zn‐β‐lactamase is active with a mono‐ and a binuclear zinc site. The apoenzyme produced by removal of both Zn ions does not recover full activity upon readdition of Zn2+ in contrast to an active mono‐Zn form prepared at pH 6.0. Differences in k cat values observed are substrate‐dependent implying distinct mechanisms for the mono‐ and binuclear species. The substrate profile of a Zn,Cd hybrid obtained by selective exchange of one zinc ion is different from that of the Zn2 enzyme with a remarkable 15‐fold increased activity with cefoxitin as substrate.Keywords
This publication has 12 references indexed in Scilit:
- 1.85 Å Resolution Structure of the ZincII β-Lactamase from Bacillus cereusActa Crystallographica Section D-Biological Crystallography, 1998
- X-ray Structure of the ZnII β-Lactamase from Bacteroides fragilis in an Orthorhombic Crystal FormActa Crystallographica Section D-Biological Crystallography, 1998
- Crystal structures of the cadmium‐ and mercury‐substituted metallo‐β‐lactamase from Bacteroides fragilisProtein Science, 1997
- Zn(II) Dependence of the Aeromonas hydrophila AE036 Metallo-β-lactamase Activity and StabilityBiochemistry, 1997
- Purification, crystallization and preliminary X-ray analysis ofBacteroides fragilisZn2+β-lactamaseActa Crystallographica Section D-Biological Crystallography, 1997
- Crystal structure of the wide-spectrum binuclear zinc β-lactamase from Bacteroides fragilisStructure, 1996
- Characterization of the Metal-Binding Sites of the β-Lactamase from Bacteroides fragilisBiochemistry, 1996
- Ligand variation and metal ion binding specificity in zinc finger peptidesInorganic Chemistry, 1993
- Biochemical characterization of the metallo-beta-lactamase CcrA from Bacteroides fragilis TAL3636Antimicrobial Agents and Chemotherapy, 1992
- Purification and characterization of an imipenem hydrolysing metallo-β-lactamase from Bacteroides fragilisJournal of Antimicrobial Chemotherapy, 1992