Receptor Signal Output Mediated by the ETR1 N Terminus Is Primarily Subfamily I Receptor Dependent
Open Access
- 4 August 2006
- journal article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 142 (2), 492-508
- https://doi.org/10.1104/pp.106.082628
Abstract
Etr1-1 is a dominant ethylene receptor gene in Arabidopsis (Arabidopsis thaliana) and confers ethylene insensitivity. The truncated etr1-1(1-349) protein is capable of repressing ethylene responses, whereas etr1(1-349) is not, lending support to a hypothesis that the dominant etr1-1(1-349) could convert wild-type receptors to an ethylene-insensitive state. Assuming that etr1-1(1-349) and etr1(1-349) would share the same signaling mechanism, we hypothesize that the etr1(1-349) protein is capable of repressing ethylene responses when not bound with ethylene. In this study, we show that both etr1(1-349) and etr1-1(1-349) are capable of receptor signal output, which is primarily dependent on subfamily I receptors. The etr1(1-349) and etr1-1(1-349) clones were individually transformed to mutants and the resulting phenotypes were scored. Each of those transgenes restored the rosette growth and flower fertility of etr1-7 ers1-2 to a similar extent. In contrast, neither etr1(1-349) nor etr1-1(1-349) was capable of signal output in etr1-7 ers1-3. The ERS1 transcript was detectable in ers1-2 but not in ers1-3, implying that ETR1 N-terminal signaling is subfamily I dependent. Loss of the subfamily II receptor genes did not perturb etr1-1(1-349)-mediated ethylene insensitivity. Possible roles of subfamily I receptors and disulfide linkages in ETR1 receptor signal output mediated through the N terminus are discussed.Keywords
This publication has 39 references indexed in Scilit:
- Nucleotide sequence, functional characterization and evolution of pFKN, a virulence plasmid in Pseudomonas syringae pathovar maculicolaMolecular Microbiology, 2003
- Biochemical and functional analysis of CTR1, a protein kinase that negatively regulates ethylene signaling inArabidopsisThe Plant Journal, 2003
- Canonical histidine kinase activity of the transmitter domain of the ETR1 ethylene receptor from Arabidopsis is not required for signal transmissionProceedings of the National Academy of Sciences of the United States of America, 2002
- Effect of Ethylene Pathway Mutations upon Expression of the Ethylene Receptor ETR1 from ArabidopsisPlant Physiology, 2002
- The two GAF domains in phosphodiesterase 2A have distinct roles in dimerization and in cGMP bindingProceedings of the National Academy of Sciences of the United States of America, 2002
- Loss-of-Function Mutations in the Ethylene ReceptorETR1 Cause Enhanced Sensitivity and Exaggerated Response to Ethylene in ArabidopsisPlant Physiology, 2002
- Mutational Analysis of the Ethylene Receptor ETR1. Role of the Histidine Kinase Domain in Dominant Ethylene InsensitivityPlant Physiology, 2002
- Arabidopsis RGL1 Encodes a Negative Regulator of Gibberellin ResponsesPlant Cell, 2002
- Two-Component Signal TransductionAnnual Review of Biochemistry, 2000
- Floral dip: a simplified method forAgrobacterium‐mediated transformation ofArabidopsis thalianaThe Plant Journal, 1998