Gibberellin biosynthesis and signalling during development of the strawberry receptacle
- 28 March 2011
- journal article
- research article
- Published by Wiley in New Phytologist
- Vol. 191 (2), 376-390
- https://doi.org/10.1111/j.1469-8137.2011.03700.x
Abstract
• The enlargement of receptacle cells during strawberry (Fragaria × ananassa) fruit development is a critical factor determining fruit size, with the increase in cell expansion being one of the most important physiological processes regulated by the phytohormone gibberellin (GA). • Here, we studied the role of GA during strawberry fruit development by analyzing the endogenous content of bioactive GAs and the expression of key components of GA signalling and metabolism. Bioactive GA1, GA3 and GA4 were monitored during fruit development, with the content of GA4 being extremely high in the receptacle, peaking at the white stage of development. • Genes with high homology to genes encoding GA pathway components, including receptors (FaGID1(GIBBERELLIN‐INSENSITIVE DWARF1)b and FaGID1c), DELLA (FaRGA(REPRESSOR OF GA) and FaGAI(GA‐INSENSITIVE)), and enzymes involved in GA biosynthesis (FaGA3ox) and catabolism (FaGA2ox), were identified, and their expression in different tissues and developmental stages of strawberry fruit was studied in detail. The expression of all of these genes showed a stage‐specific pattern during fruit development and was highest in the receptacle. FaGID1c bound GA in vitro, interacted with FaRGA in vitro and in vivo, and increased GA responses when ectopically expressed in Arabidopsis. • This study thus reveals key elements of GA responses in strawberry and points to a critical role for GA in the development of the receptacle.Keywords
This publication has 61 references indexed in Scilit:
- The Angiosperm Gibberellin-GID1-DELLA Growth Regulatory Mechanism: How an “Inhibitor of an Inhibitor” Enables Flexible Response to Fluctuating EnvironmentsPlant Cell, 2009
- Structural basis for gibberellin recognition by its receptor GID1Nature, 2008
- Reconfiguration of the Achene and Receptacle Metabolic Networks during Strawberry Fruit DevelopmentPlant Physiology, 2008
- Gibberellin Regulation of Fruit Set and Growth in TomatoPlant Physiology, 2007
- Mechanisms of Cross Talk between Gibberellin and Other HormonesPlant Physiology, 2007
- Molecular Interactions of a Soluble Gibberellin Receptor, GID1, with a Rice DELLA Protein, SLR1, and GibberellinPlant Cell, 2007
- DELLAs Contribute to Plant PhotomorphogenesisPlant Physiology, 2007
- Changes in Transcriptional Profiles Are Associated with Early Fruit Tissue Specialization in TomatoPlant Physiology, 2005
- GIBBERELLIN INSENSITIVE DWARF1 encodes a soluble receptor for gibberellinNature, 2005
- A new mathematical model for relative quantification in real-time RT-PCRNucleic Acids Research, 2001