Adiponectin secretion and response to pioglitazone is depot dependent in cultured human adipose tissue
- 1 October 2008
- journal article
- research article
- Published by American Physiological Society in American Journal of Physiology-Endocrinology and Metabolism
- Vol. 295 (4), E842-E850
- https://doi.org/10.1152/ajpendo.90359.2008
Abstract
The subcutaneous (S) and visceral (V) adipose tissue (AT) depots are increasingly recognized as distinct. To test the hypothesis that depot differences exist for adiponectin, fresh and cultured human VAT and SAT from obese type 2 diabetic (T2D) and obese nondiabetic (ND) subjects was examined to determine whether differences in adiponectin content and secretion occurred as a function of depot studied, diabetic status, and response to thiazolidinedione treatment. VAT and SAT were obtained by biopsy and AT explants cultured in defined media for 7 days. Protein expression was assessed by Western blot. Adiponectin content of conditioned medium was determined by radioimmunoassay. Diabetic status had no effect on adiponectin secretion over days 0–2 of culture. In ND SAT, secretion fell over days 2–4 but was sustained at greater levels vs. T2D SAT. In both ND and T2D VAT, adiponectin secretion was low, similar to T2D SAT. Over the 7-day culture period, cellular adiponectin increased in ND SAT and VAT; it remained unchanged in T2D SAT and VAT. Pioglitazone increased adiponectin secretion and content in all SAT. Pioglitazone failed to increase adiponectin secretion from either ND or T2D VAT and increased cellular content only in ND VAT. AT depot differences exist in the secretion of adiponectin and responsiveness to thiazolidinedione treatment. These data suggest that SAT, not VAT, appears to be the major contributor to increased circulating adipopnectin levels in response to pioglitazone treatment.Keywords
This publication has 64 references indexed in Scilit:
- Inflammation and insulin resistanceFEBS Letters, 2007
- Dysregulation of the expression and secretion of inflammation-related adipokines by hypoxia in human adipocytesPflügers Archiv - European Journal of Physiology, 2007
- Tissue-specific expression and regulation of GSK-3 in human skeletal muscle and adipose tissueAmerican Journal of Physiology-Endocrinology and Metabolism, 2006
- Pioglitazone increases secretion of high-molecular-weight adiponectin from adipocytesAmerican Journal of Physiology-Endocrinology and Metabolism, 2006
- Monocyte chemoattractant protein 1: a possible link between visceral adipose tissue-associated inflammation and subclinical echocardiographic abnormalities in uncomplicated obesityActa Endocrinologica, 2005
- Adiponectin mRNA levels in the abdominal adipose depots of nondiabetic womenInternational Journal of Obesity, 2003
- Differences in Adiponectin Protein Expression: Effect of Fat Depots and Type 2 Diabetic StatusHormone and Metabolic Research, 2002
- Adipose tissues display differential phagocytic and microbicidal activities depending on their localizationInternational Journal of Obesity, 2001
- Visceral fat as a main determinant of plasminogen activator inhibitor 1 level in womenInternational Journal of Obesity, 1998
- Impact of obesity on metabolism in men and women. Importance of regional adipose tissue distribution.JCI Insight, 1983