In Situ Embedding of Cell Monolayers Cultured on Plastic Surfaces for Electron Microscopy
- 1 January 1991
- journal article
- Published by Informa UK Limited in Biotechnic & Histochemistry
- Vol. 66 (5), 269-272
- https://doi.org/10.3109/10520299109109985
Abstract
Various procedures suitable for routine in situ embedding of cell monolayers were tested including: (1) the use of different Epon substitutes, (2) the use of different types of plasticware obtained from different sources, and (3) different methods of preparing capsules for sectioning. Different resins reacted differently with different plastics and type of preparation. Merck Epon substitute bound to most of the plastics tested. Ladd Epon substitute released cleanly from all plastics tested when a suitable method of preparation was used. The results show that for routine embedding of cell monolayers it is necessary to select an appropriate Epon substitute and method of preparation of capsules for the type of plasticware used. A routine method is described, with various alternative steps which can be applied when particular difficulties are encountered.Keywords
This publication has 14 references indexed in Scilit:
- Bone formation by osteoblast-like cells in a three-dimensional cell cultureCalcified Tissue International, 1990
- Light, scanning, and transmission electron microscopy of a single population of detergent-extracted cardiac myocytes in vitroJournal of Electron Microscopy Technique, 1989
- Sectionable microcarrier beads: An improved method for the preparation of cell monolayers for electron microscopyJournal of Electron Microscopy Technique, 1987
- A convenient method forin situ processing of cultured cells for cytochemical localization by electron microscopyThe Journal of Pathology, 1985
- Improved method for transmission electron microscopy of ciliated cell monolayers maintained on gas‐permeable membranesJournal of Microscopy, 1983
- Culture of cells in beem capsules: A new technique for electron microscopic study of monolayer culturesIn Vitro Cellular & Developmental Biology - Plant, 1976
- Vertical SectioningPublished by Elsevier BV ,1973
- A new technique for separation of coverglass substrate from epoxy-embedded specimens for electron microscopyJournal of Ultrastructure Research, 1971
- PROCEDURE FOR EMBEDDING IN SITU SELECTED CELLS CULTURED IN VITROThe Journal of cell biology, 1967
- Notes on TechnicStain Technology, 1963