Profile of the bovine acute-phase response following an intravenous bolus-dose lipopolysaccharide challenge
Open Access
- 1 April 2009
- journal article
- other
- Published by SAGE Publications in Innate Immunity
- Vol. 15 (2), 81-89
- https://doi.org/10.1177/1753425908099170
Abstract
Our objective was to characterize further the acute-phase response following endotoxin (i.e. lipopolysaccharide; LPS) exposure in the bovine. Nine pure-bred Angus castrated males (i.e. steers; average body weight = 299 ± 5 kg) were used in a randomized complete block design in environmentally controlled chambers, set at thermoneutral level, to characterize the acute physiological, endocrine, immune, and acute-phase protein responses following an i.v. bolus administration of 2.5 μg of LPS/kg body weight. One day before administration of LPS, all steers were fitted with an indwelling jugular vein catheter for serial blood collection. Blood samples were collected at 30-min intervals from -2 h to 8 h relative to the LPS challenge (time 0), and serum was harvested and stored at -80 °C until analyzed for concentrations of cortisol, pro-inflammatory cytokines, and acute-phase proteins. Indicators of thermal status (i.e. rectal temperature, ruminal temperature, respiration rate, sweat rate, and skin temperatures) were measured at 30-min intervals from -1 h to 6 h relative to the challenge. Endotoxin exposure increased (P<0.05) serum concentrations of cortisol, tumor necrosis factor-α (TNF-α), interleukin 1-β (IL-1β), IL-6, interferon-γ (IFN-γ), and serum amyloid A. Respiration rate, rectal temperature, and rump skin temperature also were increased (P<0.05) following LPS administration. Endotoxin exposure dramatically decreased ear skin temperature (P = 0.002), but tended to increase (P0.24) by the challenge. To our knowledge, this report is the most complete characterization of the bovine acute-phase response to a bolus-dose endotoxin challenge conducted under thermoneutral conditions and should provide foundation data for future research.Keywords
This publication has 14 references indexed in Scilit:
- Effects of dietary energy source and level and injection of tilmicosin phosphate on immune function in lipopolysaccharide-challenged beef steers1,2Journal of Animal Science, 2008
- Exogenous testosterone modulates tumor necrosis factor-α and acute phase protein responses to repeated endotoxin challenge in steersDomestic Animal Endocrinology, 2006
- Dose Dependency and Individual Variability of the Lipopolysaccharide-Induced Bovine Acute Phase Protein ResponseJournal of Dairy Science, 2004
- Effect of Lipopolysaccharide on Indices of Peripheral and Hepatic Metabolism in Lactating CowsJournal of Dairy Science, 2003
- The concept of sickness behavior: a brief chronological account of four key discoveriesVeterinary Immunology and Immunopathology, 2002
- The metabolic consequences of stress: targets for stress and priorities of nutrient use.Published by CABI Publishing ,2000
- Interaction of cattle health/immunity and nutrition.Journal of Animal Science, 1999
- Primary bovine hepatocytes in the study of cytokine induced acute-phase protein secretion in vitroVeterinary Immunology and Immunopathology, 1996
- Concentrations of serum Amyloid‐a (SAA) and haptoglobin (HP) as parameters of inflammatory diseases in cattleVeterinary Quarterly, 1994
- Bovine and canine acute phase proteinsVeterinary Research Communications, 1988