MicroRNA‐130a regulates cerebral ischemia–induced blood–brain barrier permeability by targeting Homeobox A5
Open Access
- 3 January 2018
- journal article
- research article
- Published by Wiley in The FASEB Journal
- Vol. 32 (2), 935-944
- https://doi.org/10.1096/fj.201700139rrr
Abstract
Blood–brain barrier (BBB) disruption plays a critical role in brain injury induced by cerebral ischemia, and preserving BBB integrity during ischemia could alleviate cerebral injury. We examined the role of miR-130a in ischemic BBB disruption by using models of rat middle cerebral artery occlusion and cell oxygen–glucose deprivation. We found that ischemia significantly increased microRNA-130a (miR-130a) level and that miR-130a was predominantly from brain microvascular endothelial cells. Antagomir-130a, an antagonist of miR-130a, could attenuate brain edema, lower BBB permeability, reduce infarct volume, and improve neurologic function. MiR-130a overexpression induced by miR-130a mimic increased monolayer permeability, and intercellular inhibition of miR-130a by a miR-130a inhibitor suppressed oxygen–glucose deprivation–induced increase in monolayer permeability. Moreover, dual luciferase reporter system showed that Homeobox A5 was the direct target of miR-130a. MiR-130a, by inhibiting Homeobox A5 expression, could down-regulate occludin, thereby increasing BBB permeability. Our results suggested that miR-130a might be implicated in ischemia-induced BBB dysfunction and serve as a target for the treatment of ischemic stroke.—Wang, Y., Wang, M.-D., Xia, Y.-P., Gao, Y., Zhu, Y.-Y., Chen, S.-C., Mao, L., He, Q.-W., Yue, Z.-Y., Hu, B. MicroRNA-130a regulates cerebral ischemia–induced blood–brain barrier permeability by targeting Homeobox A5. FASEB J. 32, 935–944 (2018). www.fasebj.orgKeywords
Funding Information
- National Natural Science Foundation of China (81301001, 81571119, 81371311, 81571139, 81671147, 81301002, 81400969, 81400970, 81601027)
- National Basic Research Program of China (2016YFC1300600)
- Program for New Century Excellent Talents in University
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