Usefulness of Published PCR Primers in Detecting Human Rhinovirus Infection
Open Access
- 1 February 2011
- journal article
- research article
- Published by Centers for Disease Control and Prevention (CDC) in Emerging Infectious Diseases
- Vol. 17 (2), 296-298
- https://doi.org/10.3201/eid1702.101123
Abstract
We conducted a preliminary comparison of the relative sensitivity of a cross-section of published human rhinovirus (HRV)–specific PCR primer pairs, varying the oligonucleotides and annealing temperature. None of the pairs could detect all HRVs in 2 panels of genotyped clinical specimens; >1 PCR is required for accurate description of HRV epidemiology.Keywords
This publication has 14 references indexed in Scilit:
- Newly identified respiratory viruses in children with asthma exacerbation not requiring admission to hospitalJournal of Medical Virology, 2010
- New Respiratory Enterovirus and Recombinant Rhinoviruses among Circulating PicornavirusesEmerging Infectious Diseases, 2009
- Real-Time Reverse Transcription-PCR Assay for Comprehensive Detection of Human RhinovirusesJournal of Clinical Microbiology, 2008
- Community Epidemiology of Human Metapneumovirus, Human Coronavirus NL63, and Other Respiratory Viruses in Healthy Preschool-Aged Children Using Parent-Collected SpecimensPediatrics, 2007
- Frequent detection of human rhinoviruses, paramyxoviruses, coronaviruses, and bocavirus during acute respiratory tract infectionsJournal of Medical Virology, 2006
- Picornavirus infections in children diagnosed by RT-PCR during longitudinal surveillance with weekly sampling: Association with symptomatic illness and effect of seasonJournal of Medical Virology, 2006
- Real-time RT-PCR detection of 12 respiratory viral infections in four triplex reactionsJournal of Clinical Virology, 2005
- Simultaneous detection of fourteen respiratory viruses in clinical specimens by two multiplex reverse transcription nested‐PCR assaysJournal of Medical Virology, 2004
- Early Detection of Acute Rhinovirus Infections by a Rapid Reverse Transcription-PCR AssayJournal of Clinical Microbiology, 2001
- Amplification of rhinovirus specific nucleic acids from clinical samples using the polymerase chain reactionJournal of Medical Virology, 1989