Triplet-state monitoring by fluorescence correlation spectroscopy
- 1 September 1994
- journal article
- Published by Springer Science and Business Media LLC in Journal of Fluorescence
- Vol. 4 (3), 255-258
- https://doi.org/10.1007/bf01878460
Abstract
The effects of high excitation intensities in fluorescence correlation spectroscopy (FCS) in terms of saturation and triplet-state build-up have been studied for the case of Rh6G in aqueous solution. It was found that FCS provides a powerful means for the determination of intersystem crossing and triplet-state depopulation rates of fluorophores in solution.Keywords
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