cDNA cloning, characterization, and variation analysis of chicken adipose triglyceride lipase (ATGL) gene

Abstract

Adipose triglyceride lipase (ATGL) is an important triglyceride-specific lipase that catalyzes the initial step in triglyceride hydrolysis. In this study, cloning, sequencing, and mRNA real-time analyses were employed to characterize the chicken ATGL gene. We obtained a total of 1,528-bp long chicken ATGL cDNA fragment including 51-bp 5′UTR, 1,452-bp open reading frame (ORF), and 25-bp 3′UTR. The predicted chicken ATGL had 483 amino acids and a molecular weight of 53.5 kDa, giving rise to identities of 66.5%, 67.3%, 68.2%, 64.8%, and 66.5% with that of human, mouse, rat, pig, and cattle, respectively. The chicken ATGL gene spanned over 30,197 bp and comprised of nine exons and eight introns, in which the intron 1 (21,146 bp) was far longer than others. It predominantly expressed in subcutaneous fat and abdominal fat and then in kidney and lung. Very low but detectable mRNA level was also observed in other 15 tissues. However, no mRNA was detected in spleen. A total of 15 single nucleotide polymorphisms (SNPs) were identified in its complete cDNA sequences with an average of one SNP in every 102 bp and a summarized nucleotide diversity of 3.02 × 10⁻³. Seven of the 15 SNPs were non-synonymous. All SNPs had allelic frequencies over 5% and could be considered as candidate markers for future marker-trait association analysis.