Antibodies against Linear Epitopes on the Goodpasture Autoantigen and Kidney Injury
- 1 June 2012
- journal article
- Published by Ovid Technologies (Wolters Kluwer Health) in Clinical Journal of the American Society of Nephrology
- Vol. 7 (6), 926-933
- https://doi.org/10.2215/cjn.09930911
Abstract
Background and objectives Linear epitopes on the Goodpasture autoantigen involved in human anti-glomerular basement membrane (GBM) disease are not fully defined. This study investigated the linear epitopes recognized by circulating antibodies in anti-GBM patients, aiming to identify the potential nephrogenic linear epitopes and their clinical significance. Design, setting, participants, & measurements Sixty-eight patients with anti-GBM disease were enrolled. Twenty-four overlapping linear peptides were synthesized across the whole sequence of the human Goodpasture autoantigen. ELISA detected circulating antibodies against linear epitopes. Their associations with clinical features were further analyzed. Results Antibodies against linear peptides were detected in sera from 55 patients (80.9%). Three major epitopes with high frequencies were identified: P14 (41%), P16 (36.8%), and P18 (57%). P14, a formerly defined T cell epitope, was a mutual B cell epitope. Antibodies against P14 were frequently detected in patients with positive antineutrophil cytoplasmic antibodies (39.3% versus 12.5%; P=0.01). Patients with anti-P16 antibodies presented with higher serum creatinine on diagnosis (665.5±227.2 versus 443.7±296.8 μmol/L; P=0.001) and worse renal outcome during follow-up (hazard ratio, 2.10; 95% confidence interval, 1.10–3.90; P=0.02). The level of anti-P18 antibodies positively correlated with the percentage of crescents in glomeruli (r=0.54; P=0.008). Recognition of P22 was an independent predictor for patient death (hazard ratio, 3.02; 95% confidence interval, 1.20–7.57; P=0.02). Conclusions Antibodies against linear epitopes on the Goodpasture autoantigen could be detected in human anti-GBM disease and were associated with kidney injury. P14 was a mutual T and B cell epitope, implying its nephrogenic role in disease initiation.Keywords
This publication has 39 references indexed in Scilit:
- Clinical Features and Outcomes of Anti–Glomerular Basement Membrane Disease in Older PatientsAmerican Journal of Kidney Diseases, 2011
- Molecular Architecture of the Goodpasture Autoantigen in Anti-GBM NephritisThe New England Journal of Medicine, 2010
- A Sulfilimine Bond Identified in Collagen IVScience, 2009
- Levels of epitope-specific autoantibodies correlate with renal damage in anti-GBM diseaseNephrology Dialysis Transplantation, 2009
- Identification of a nephritogenic immunodominant B and T cell epitope in experimental autoimmune glomerulonephritisClinical and Experimental Immunology, 2008
- High nephritogenicity of monoclonal antibodies belonging to IgG2a and IgG2b subclasses in rat anti-GBM nephritisKidney International, 2004
- Presentation of the Goodpasture Autoantigen to CD4 T Cells Is Influenced More by Processing Constraints Than by HLA Class II Peptide Binding PreferencesPublished by Elsevier BV ,1998
- Direct Identification of Naturally Processed Autoantigen-derived Peptides Bound to HLA-DR15Published by Elsevier BV ,1996
- Molecular cloning of the human Goodpasture antigen demonstrates it to be the alpha 3 chain of type IV collagen.JCI Insight, 1992
- Perfusion Experiments in the Study of Cellular AnaphylaxisThe American Journal of the Medical Sciences, 1919