Untersuchungen über den Stoffwechsel der Aminosäuren im Tierkörper. Mit 2 Figuren im Text.

Abstract

The tissue slice method of Warburg was used in the study of the metabolism of aminoacids. Thin slices (2-20 mgm.) chiefly from rat-organs, but also from rabbits, cats, dogs and human beings, were employed in a physiological salt solution buffered at pH = 7.4; pieces of the diaphragm, retina and gut were used in original thickness. O2 consumption was determined manometrically over periods of 1-2 hrs. Amino-N was estimated according to van Slyke, ammonia according to Parnas and keto-acids according to the manometric method, or the method of isolation of Neuberg and Kobel. Decomposition of amino-acids takes place in the kidneys as well as in the liver. The NH3 content of the urine can therefore be derived from amino-acids. In the slices of kidneys (rats) deamination of optical exantiomorphs of natural amino acids takes place 10-30 times more easily than that of the natural amino acids themselves. The deamination of amino acids is almost quantitatively an oxidative process in which keto-aeids are produced. The deamination does not take place under anaerobic conditions.