First report of pathogenic Leptospira spp. isolated from urine and kidneys of naturally infected cats
Open Access
- 10 March 2020
- journal article
- research article
- Published by Public Library of Science (PLoS) in PLOS ONE
- Vol. 15 (3), e0230048
- https://doi.org/10.1371/journal.pone.0230048
Abstract
Leptospirosis is one of the most widespread zoonotic diseases and can infect both humans and animals worldwide. Healthy cat, as a potential source of exposure to humans, are likely underestimated owing to the lack of overt clinical signs associated with Leptospira spp. infection in this species. The aim of the study was to determine the exposure, shedding, and carrier status of leptospires in shelter cats in Malaysia by using serological, molecular, and bacteriological methods. For this study, 82 healthy cats from two shelters were sampled. The blood, urine, and kidneys were tested using the microscopic agglutination test (MAT), polymerase chain reaction (PCR), and bacterial culture. On the basis of serological, molecular, and/or culture techniques, the total detection of leptospiral infection was 29.3% (n = 24/82). Through culture techniques, 16.7% (n = 4/24) of the cats that tested positive were carriers with positive kidney cultures, and one cat was culture positive for both urine and kidney. The Leptospira spp. isolates were identified as pathogenic L. interrogans serovar Bataviae through serological and molecular methods. Through serological techniques, 87.5% (n = 21/24) had positive antibody titers (100–1600) and most of the Bataviae serogroup (n = 19/21). Using PCR, 16.7% (n = 4/24) of cats were shown to have pathogenic Leptospira spp. DNA in their urine. Furthermore, three out of four culture positive cats were serology negative. The present study reports the first retrieval of pathogenic leptospires from urine and kidneys obtained from naturally infected cats. The results provide evidence of the potential role of naturally infected cats in the transmission of leptospires. Additionally, leptospiral infection occurs sub-clinically in cats. The culture isolation provides evidence that healthy cats could be reservoirs of leptospiral infection, and this information may promote the development of disease prevention strategies for the cat population.Keywords
Funding Information
- Universiti Putra Malaysia (GP-IPS/2018/9641800)
This publication has 42 references indexed in Scilit:
- Rats, Cities, People, and Pathogens: A Systematic Review and Narrative Synthesis of Literature Regarding the Ecology of Rat-Associated Zoonoses in Urban CentersVector-Borne and Zoonotic Diseases, 2013
- The Prevalence of Leptospiral Antibodies in Free Roaming Cats in Worcester County, MassachusettsJournal of Veterinary Internal Medicine, 2012
- Emergence, control and re-emerging leptospirosis: dynamics of infection in the changing worldClinical Microbiology & Infection, 2011
- Cross-Species Surveillance of Leptospira in Domestic and Peri-Domestic Animals in Mahalla City, Gharbeya Governorate, EgyptThe American Journal of Tropical Medicine and Hygiene, 2011
- Diagnostic Accuracy of Real-Time PCR Assays Targeting 16S rRNA and lipl32 Genes for Human Leptospirosis in Thailand: A Case-Control StudyPLOS ONE, 2011
- 2010 ACVIM Small Animal Consensus Statement on Leptospirosis: Diagnosis, Epidemiology, Treatment, and PreventionJournal of Veterinary Internal Medicine, 2010
- Leptospira as an emerging pathogen: a review of its biology, pathogenesis and host immune responsesFuture Microbiology, 2010
- Leptospira: the dawn of the molecular genetics era for an emerging zoonotic pathogenNature Reviews Microbiology, 2009
- Species-Specific Identification of Leptospiraceae by 16S rRNA Gene SequencingJournal of Clinical Microbiology, 2006
- Leptospira DNA detection for the diagnosis of human leptospirosisJournal of Infection, 2006