Peptide mimics selected from immune sera using phage display technology can replace native antigens in the diagnosis of Epstein-Barr virus infection
Open Access
- 24 November 2008
- journal article
- research article
- Published by Oxford University Press (OUP) in Protein Engineering, Design and Selection
- Vol. 22 (2), 85-91
- https://doi.org/10.1093/protein/gzn076
Abstract
There is an expanding area of small molecule discovery, especially in the area of peptide mimetics. Peptide sequences can be used to substitute for the entire native antigen for use in diagnostic assays. Our approach is to select peptides that mimic epitopes of the natural immune response to Epstein–Barr virus (EBV) that may be recognised by antibodies typically produced after infection with EBV. We screened a random peptide library on sera from rabbits immunised with a crude preparation of EBV and serum antibodies from a patient with a high titer of EBV antibodies. We selected four peptides (Eb1–4) with the highest relative binding affinity with immune rabbit sera and a single peptide with high affinity to human serum antibodies. The peptides were coupled to the carrier molecule BSA and the recognition of the peptides by IgM antibodies in clinical samples after infection with EBV was measured. The sensitivities were Eb1 94%, Eb2, 3, 4 88%, H1 81% and all had 100% specificity. This study illustrates that the phage display approach to select epitope mimics can be applied to polyclonal antibodies and peptides that represent several diagnostically important epitopes can be selected simultaneously. This panel of EBV peptides representing a wide coverage of immunodominant epitopes could replace crude antigen preparations currently used for capture in commercial diagnostic tests for EBV.Keywords
This publication has 11 references indexed in Scilit:
- Display of a Peptide Mimotope on a Crystalline Bacterial Cell Surface Layer (S-layer) Lattice for Diagnosis of Epstein–Barr Virus InfectionBioconjugate Chemistry, 2008
- Phage Display of Peptides in Ligand Selection for Use in Affinity ChromatographyPublished by Springer Science and Business Media LLC ,2008
- Identification of hepatitis A virus mimotopes by phage display, antigenicity and immunogenicityJournal of Virological Methods, 2007
- Peptide Mimotopes Selected from a Random Peptide Library for Diagnosis of Epstein-Barr Virus InfectionJournal of Clinical Microbiology, 2006
- EBV and Systemic Lupus Erythematosus: A New PerspectiveThe Journal of Immunology, 2005
- Routine Epstein-Barr Virus Diagnostics from the Laboratory Perspective: Still Challenging after 35 YearsJournal of Clinical Microbiology, 2004
- Antibodies to Malaria Peptide Mimics Inhibit Plasmodium falciparum Invasion of ErythrocytesInfection and Immunity, 2004
- Evaluation of Four Commercially Available Epstein-Barr Virus Enzyme Immunoassays with an Immunofluorescence Assay as the Reference MethodClinical and Vaccine Immunology, 2003
- Fingerprinting the circulating repertoire of antibodies from cancer patientsNature Biotechnology, 2002
- The EB VirusAnnual Review of Microbiology, 1973