Analysis of 1H/2H Exchange Kinetics Using Model Infrared Spectra
- 1 January 2004
- journal article
- research article
- Published by SAGE Publications in Applied Spectroscopy
- Vol. 58 (1), 68-82
- https://doi.org/10.1366/000370204322729496
Abstract
This paper investigates the different approaches that best retrieve band shape parameters and kinetic time constants from series of protein Fourier transform infrared (FT-IR) spectra recorded in the course of 1H/2H exchange. In this first approach, synthetic spectra were used. It is shown that 1H/2H exchange kinetic measurements can help resolve spectral features otherwise hidden because of the overlap of various spectral contributions. We evaluated the efficiency of Fourier self-deconvolution, synchronous/asynchronous correlation, difference spectroscopy, principal component analysis, inverse Laplace transform, and determination of the underlying spectra by global analysis assuming first-order kinetics with either known or unknown time constants. It is demonstrated that some strategies allow the extraction of both the time dependence and the spectral shape of the underlying contributions.Keywords
This publication has 52 references indexed in Scilit:
- New Approach to Generalized Two-Dimensional Correlation Spectroscopy. 1: Combination of Principal Component Analysis and Two-Dimensional Correlation SpectroscopyApplied Spectroscopy, 2002
- Monitoring Structural Stability of Trypsin Inhibitor at the Submolecular Level by Amide−Proton Exchange Using Fourier Transform Infrared Spectroscopy: A Test Case for More General ApplicationBiochemistry, 1997
- Two-Dimensional FT-IR Spectroscopy: A Powerful Method to Study the Secondary Structure of Proteins Using H-D ExchangeApplied Spectroscopy, 1997
- Quantitative Two-Dimensional Infrared (2D IR) Spectroscopy: Theoretical Development for General and Specific CasesApplied Spectroscopy, 1995
- Fourier Transform Infrared Spectroscopy Study of the Secondary Structure of the Reconstituted Neurospora crassa Plasma Membrane H+-ATPase and of Its Membrane-associated Proteolytic PeptidesPublished by Elsevier BV ,1995
- Secondary structure and dosage of soluble and membrane proteins by attenuated total reflection Fourier‐transform infrared spectroscopy on hydrated filmsEuropean Journal of Biochemistry, 1990
- Quantitative IR spectrophotometry of peptide compounds in water (H2O) solutions. II. Amide absorption bands of polypeptides and fibrous proteins in α‐, β‐, and random coil conformationsPeptide Science, 1990
- CONTIN: A general purpose constrained regularization program for inverting noisy linear algebraic and integral equationsComputer Physics Communications, 1982
- Fourier transforms in the computation of self-deconvoluted and first-order derivative spectra of overlapped band contoursAnalytical Chemistry, 1981
- Noise in Fourier self-deconvolutionApplied Optics, 1981