Arabidopsis RAV1 is down-regulated by brassinosteroid and may act as a negative regulator during plant development
Open Access
- 1 February 2004
- journal article
- research article
- Published by Springer Science and Business Media LLC in Cell Research
- Vol. 14 (1), 8-15
- https://doi.org/10.1038/sj.cr.7290197
Abstract
RAV1 is a novel DNA-binding protein with two distinct DNA-binding domains unique in higher plants, but its role in plant growth and development remains unknown. Using cDNA array, we found that transcription of RAV1 is down-regulated by epibrassinolide (epiBL) in Arabidopsis suspension cells. RNA gel blot analysis revealed that epiBL-regulated RAV1 transcription involves neither protein phosphorylation/dephosphorylation nor newly synthesized protein, and does not require the functional BRI1, suggesting that this regulation might be through a new BR signaling pathway. Overexpressing RAV1 in Arabidopsis results in a retardation of lateral root and rosette leaf development, and the underexpression causes an earlier flowering phenotype, implying that RAV1 may function as a negative regulatory component of growth and development.Keywords
This publication has 47 references indexed in Scilit:
- Brassinosteroid-Regulated Gene ExpressionPlant Physiology, 2002
- DNA-Binding Specificity of the ERF/AP2 Domain of Arabidopsis DREBs, Transcription Factors Involved in Dehydration- and Cold-Inducible Gene ExpressionBiochemical and Biophysical Research Communications, 2002
- High-Molecular-Weight FK506-Binding Proteins Are Components of Heat-Shock Protein 90 Heterocomplexes in Wheat Germ LysatePlant Physiology, 1998
- A Dissociation insertion causes a semidominant mutation that increases expression of TINY, an Arabidopsis gene related to APETALA2.Plant Cell, 1996
- Binding of Immunophilins to the 90 kDa Heat Shock Protein (hsp90) via a Tetratricopeptide Repeat Domain Is a Conserved Protein Interaction in PlantsBiochemistry, 1996
- Animal and Plant Cell Lysates Share a Conserved Chaperone System That Assembles the Glucocorticoid Receptor into a Functional Heterocomplex with hsp90Biochemistry, 1996
- Nongenomic Steroid Action: Independent Targeting of a Plasma Membrane Calcium Channel and a Tyrosine KinaseBiochemical and Biophysical Research Communications, 1995
- The APETALA2 domain is related to a novel type of DNA binding domain.Plant Cell, 1995
- Arabidopsis phosphoribosylanthranilate isomerase: molecular genetic analysis of triplicate tryptophan pathway genes.Plant Cell, 1995
- Molecular Cloning and Characterization of a Brassinosteroid-Regulated Gene from Elongating Soybean (Glycine max L.) EpicotylsPlant Physiology, 1994