Difference in metabolite levels between photoautotrophic and photomixotrophic cultures of Synechocystis sp. PCC 6803 examined by capillary electrophoresis electrospray ionization mass spectrometry
Open Access
- 13 June 2008
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of Experimental Botany
- Vol. 59 (11), 3009-3018
- https://doi.org/10.1093/jxb/ern157
Abstract
Capillary electrophoresis mass spectrometry (CE/MS) was applied for the comprehensive survey of changes in the amounts of metabolites upon the shift from photoautotrophic to photomixotrophic conditions in Synechocystis sp. PCC 6803. When glucose was added to the photoautotrophically grown culture, the increase in the metabolites for the oxidative pentose phosphate (OPP) pathway and glycolysis, together with the decrease in those for the Calvin cycle, was observed. Concomitantly, the increase in respiratory activity and the decrease in photosynthetic activity took place in the wild-type cells. In the pmgA-disrupted mutant that shows growth inhibition under photomixotrophic conditions, lower enzymatic activities of the OPP pathway and higher photosynthetic activity were observed, irrespective of trophic conditions. These defects brought about metabolic disorders such as a decrease in ATP and NADPH contents, a failure in the activation of respiratory activity, and the aberrant accumulation of isocitrate under photomixotrophic but not under photoautotrophic conditions. A delicate balancing of the carbon flow between the Calvin cycle and the OPP pathway seems indispensable for growth specifically under photomixotrophic conditions and PmgA is likely to be involved in the regulation.Keywords
This publication has 26 references indexed in Scilit:
- Evaluation of Metabolic Alteration in Transgenic Rice Overexpressing Dihydroflavonol-4-reductaseAnnals of Botany, 2006
- A putative sensor kinase, Hik31, is involved in the response of Synechocystis sp. strain PCC 6803 to the presence of glucoseMicrobiology, 2006
- The Calvin cycle in cyanobacteria is regulated by CP12 via the NAD(H)/NADP(H) ratio under light/dark conditionsThe Plant Journal, 2005
- Pleiotropic Effect of a Histidine Kinase on Carbohydrate Metabolism in Synechocystis sp. Strain PCC 6803 and Its Requirement for Heterotrophic GrowthJournal of Bacteriology, 2005
- Towards Functional Proteomics of Membrane Protein Complexes in Synechocystis sp. PCC 6803Plant Physiology, 2004
- Genome-Wide Dynamic Transcriptional Profiling of the Light-to-Dark Transition inSynechocystissp. Strain PCC 6803Journal of Bacteriology, 2002
- Quantitative analysis of intracellular metabolic fluxes using GC-MS and two-dimensional NMR spectroscopyJournal of Bioscience and Bioengineering, 2002
- Fermentation in cyanobacteriaFEMS Microbiology Reviews, 1997
- Generic Assignments, Strain Histories and Properties of Pure Cultures of CyanobacteriaMicrobiology, 1979
- The Incomplete Tricarboxylic Acid Cycle in the Blue-green Alga Anabaena VariabilisJournal of General Microbiology, 1969