Identification ofEscherichia coliYgaF as anl-2-Hydroxyglutarate Oxidase
- 1 June 2008
- journal article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 190 (11), 3793-3798
- https://doi.org/10.1128/jb.01977-07
Abstract
YgaF, a protein of previously unknown function in Escherichia coli, was shown to possess noncovalently bound flavin adenine dinucleotide and to exhibit L-2-hydroxyglutarate oxidase activity. The inability of anaerobic, reduced enzyme to reverse the reaction by reducing the product alpha-ketoglutaric acid is explained by the very high reduction potential (+19 mV) of the bound cofactor. The likely role of this enzyme in the cell is to recover alpha-ketoglutarate mistakenly reduced by other enzymes or formed during growth on propionate. On the basis of the identified function, we propose that this gene be renamed lhgO.Keywords
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