Determination of ochratoxin A in regional samples of cow's milk from Germany
- 1 August 1996
- journal article
- research article
- Published by Informa UK Limited in Food Additives & Contaminants
- Vol. 13 (6), 669-676
- https://doi.org/10.1080/02652039609374452
Abstract
Samples (121) of cow's milk from a northern region of Germany were analysed for the occurrence of ochratoxin A by means of a sensitive high-performance liquid chromatographic (HPLC) method. The samples were extracted with a mixture of chloroform and methanol at pH < 2. The extracts were cleaned up by solid-phase extraction on silica gel cartridges. The detection limit was 0.01 ng/ml, the quantitation limit was estimated at 0.03 ng/ml. The mean recovery from spiked samples was 84 +/- 7% in the concentration range of 0.03-0.5 ng/ml. An enzyme-linked immunosorbent-assay (ELISA) was shown to be suitable for the confirmation of ochratoxin A levels down to the detection limit of the HPLC method. No ochratoxin A was detected in the samples analysed, either by HPLC or by ELISA.Keywords
This publication has 13 references indexed in Scilit:
- Determination of ochratoxin A in bile of swine by high-performance liquid chromatographyJournal of Chromatography B: Biomedical Sciences and Applications, 1995
- Determination of ochratoxin A at the ppt level in human blood, serum, milk and some foodstuffs by high-performance liquid chromatography with enhanced fluorescence detection and immunoaffinity column cleanup: methodology and Swiss dataJournal of Chromatography B: Biomedical Sciences and Applications, 1995
- Determination of ochratoxin A in urine and faeces of swine by high-performance liquid chromatographyJournal of Chromatography B: Biomedical Sciences and Applications, 1993
- A review of recent advances in understanding ochratoxicosisJournal of Animal Science, 1992
- Studies on the Application of Enzyme Immunoassays for the Fusarium Mycotoxins Deoxynivalenol, 3‐Acetyldeoxynivalenol, and ZearalenoneJournal of Veterinary Medicine, Series B, 1992
- Plasma ochratoxin A levels in three Swedish populations surveyed using an ion‐pair HPLC techniqueFood Additives & Contaminants, 1991
- Minimal transmission of zearalenone to milk of dairy cowsJournal of Environmental Science and Health, Part B, 1990
- Determination of ochratoxin A in human milkZeitschrift für Lebensmittel-Untersuchung und Forschung, 1988
- Ochratoxin A in der NahrungsmittelketteJournal of Veterinary Medicine, Series B, 1987
- The ‘carry-over’ of aflatoxin, ochratoxin and zearalenone from naturally contaminated feed to tissues, urine and milk of dairy cowsFood and Cosmetics Toxicology, 1979