Fucose-Containing Sulfated Polysaccharides from Brown Seaweeds Inhibit Proliferation of Melanoma Cells and Induce Apoptosis by Activation of Caspase-3 in Vitro
Open Access
- 13 December 2011
- journal article
- research article
- Published by MDPI AG in Marine Drugs
- Vol. 9 (12), 2605-2621
- https://doi.org/10.3390/md9122605
Abstract
Fucose-containing sulfated polysaccharides (FCSPs) extracted from seaweeds, especially brown macro-algae, are known to possess essential bioactive properties, notably growth inhibitory effects on tumor cells. In this work, we conducted a series of in vitro studies to examine the influence of FCSPs products from Sargassum henslowianum C. Agardh (FSAR) and Fucus vesiculosus (FVES), respectively, on proliferation of melanoma B16 cells and to investigate the underlying apoptosis promoting mechanisms. Cell viability analysis showed that both FCSPs products, i.e., FSAR and FVES, decreased the proliferation of the melanoma cells in a dose-response fashion, with FSAR being more potent at lower dosages, and FVES being relatively more anti-proliferative than FSAR at higher dosages. Flow cytometric analysis by Annexin V staining of the melanoma cells exposed to the FCSPs products confirmed that both FSAR and FVES induced apoptosis. The FCSPs-induced apoptosis was evidenced by loss of plasma membrane asymmetry and translocation of the cell membrane phospholipids and was accompanied by the activation of caspase-3. The FCSPs bioactivity is proposed to be attributable to distinct structural features of the FCSPs, particularly the presence of sulfated galactofucans (notably in S. henslowianum) and sulfated fucans (notably in F. vesiculosus). This study thus indicates that unfractionated FCSPs may exert bioactive effects on skin cancer cells via induction of apoptosis through cascades of reactions that involve activation of caspase-3.Keywords
This publication has 38 references indexed in Scilit:
- Important Determinants for Fucoidan Bioactivity: A Critical Review of Structure-Function Relations and Extraction Methods for Fucose-Containing Sulfated Polysaccharides from Brown SeaweedsMarine Drugs, 2011
- Heterofucan from Sargassum filipendula Induces Apoptosis in HeLa CellsMarine Drugs, 2011
- Fucoidan present in brown algae induces apoptosis of human colon cancer cellsBMC Gastroenterology, 2010
- Structure and antitumour activity of fucoidan isolated from sporophyll of Korean brown seaweed Undaria pinnatifidaCarbohydrate Polymers, 2010
- Fucoidan Induces Apoptosis through Activation of Caspase-8 on Human Breast Cancer MCF-7 CellsJournal of Agricultural and Food Chemistry, 2009
- Antiretroviral activity of fucoidans extracted from the brown seaweed Adenocystis utricularisPhytotherapy Research, 2008
- Fucoidan induces apoptosis of human HS-Sultan cells accompanied by activation of caspase-3 and down-regulation of ERK PathwaysAmerican Journal of Hematology, 2004
- Sulfated fucans, fresh perspectives: structures, functions, and biological properties of sulfated fucans and an overview of enzymes active toward this class of polysaccharideGlycobiology, 2003
- Effect of dietary seaweed preparations on 1,2-dimethylhydrazine-induced intestinal carcinogenesis in ratsCancer Letters, 1985
- Sugar constituents of sulfated polysaccharides from the fronds of Sargassum ringgoldianum.NIPPON SUISAN GAKKAISHI, 1982