Fluorescent Hydroxylamine Derived from the Fragmentation of PAMAM Dendrimers for Intracellular Hypochlorite Recognition
- 12 July 2013
- journal article
- research article
- Published by Wiley in Chemistry – A European Journal
- Vol. 19 (35), 11672-11675
- https://doi.org/10.1002/chem.201300494
Abstract
Herein, a promising sensing approach based on the structure fragmentation of poly(amidoamine) (PAMAM) dendrimers for the selective detection of intracellular hypochlorite (OCl−) is reported. PAMAM dendrimers were easily disrupted by a cascade of oxidations in the tertiary amines of the dendritic core to produce an unsaturated hydroxylamine with blue fluorescence. Specially, the novel fluorophore was only sensitive to OCl−, one of reactive oxygen species (ROS), resulting in an irreversible fluorescence turn-off. The fluorescent hydroxylamine was selectively oxidised by OCl− to form a labile oxoammonium cation that underwent further degradation. Without using any troublesomely synthetic steps, the novel sensing platform based on the fragmentation of PAMAM dendrimers, can be applied to detect OCl− in macrophage cells. The results suggest that the sensing approach may be useful for the detection of intracellular OCl− with minimal interference from biological matrixes.Keywords
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