3D DNA Origami Crystals
Top Cited Papers
- 12 July 2018
- journal article
- research article
- Published by Wiley in Advanced Materials
- Vol. 30 (28), e1800273
- https://doi.org/10.1002/adma.201800273
Abstract
3D crystals assembled entirely from DNA provide a route to design materials on a molecular level and to arrange guest particles in predefined lattices. This requires design schemes that provide high rigidity and sufficiently large open guest space. A DNA-origami-based tensegrity triangle structure that assembles into a 3D rhombohedral crystalline lattice with an open structure in which 90% of the volume is empty space is presented here. Site-specific placement of gold nanoparticles within the lattice demonstrates that these crystals are spacious enough to efficiently host 20 nm particles in a cavity size of 1.83 x 10(5) nm(3), which would also suffice to accommodate ribosome-sized macromolecules. The accurate assembly of the DNA origami lattice itself, as well as the precise incorporation of gold particles, is validated by electron microscopy and small-angle X-ray scattering experiments. The results show that it is possible to create DNA building blocks that assemble into lattices with customized geometry. Site-specific hosting of nano objects in the optically transparent DNA lattice sets the stage for metamaterial and structural biology applications.Keywords
Funding Information
- Deutsche Forschungsgemeinschaft (1032)
- European Research Council (336440)
- National Science Foundation (DMR‐0520547)
This publication has 45 references indexed in Scilit:
- Dynamic DNA devices and assemblies formed by shape-complementary, non–base pairing 3D componentsScience, 2015
- Programmable molecular recognition based on the geometry of DNA nanostructuresNature Chemistry, 2011
- From molecular to macroscopic via the rational design of a self-assembled 3D DNA crystalNature, 2009
- Self-assembly of DNA into nanoscale three-dimensional shapesNature, 2009
- Folding DNA to create nanoscale shapes and patternsNature, 2006
- DNA in a material worldNature, 2003
- Self-Assembly at All ScalesScience, 2002
- A DNA-based method for rationally assembling nanoparticles into macroscopic materialsNature, 1996
- DNA double-crossover moleculesBiochemistry, 1993
- Nucleic acid junctions and latticesJournal of Theoretical Biology, 1982