Direct Measurement of Torque in an Optical Trap and Its Application to Double-Strand DNA

Abstract

We present a method that offers the possibility to directly apply and measure torque on particles in an optical trap. It can be used to rotationally manipulate biopolymers attached to appropriate particles. A flat object is trapped and oriented in the focus of a linearly polarized laser light. The direction and power of the orientational trap are controlled by the polarization state of the light. As a demonstration of the capabilities of the method, we examined the torsional stiffness of dsDNA ($\lambda$-DNA) in its linear torsional regime by directly measuring the torque generated by the molecule. DOI: http://dx.doi.org/10.1103/PhysRevLett.97.058301 © 2006 The American Physical Society