Carbon Assimilation Profiles as a Tool for Identification of Zygomycetes
- 1 May 2007
- journal article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 45 (5), 1433-1439
- https://doi.org/10.1128/jcm.02219-06
Abstract
Identification of Zygomycetes is difficult and time-consuming by standard microbiological procedures. Carbon assimilation profiles are commonly used for yeast-and bacterial-species identification but rarely for filamentous-fungus identification. Carbon assimilation profiles were evaluated using the commercialized kits ID32C and API 50 CH, which contain 31 and 49 tests, respectively, to serve as simple tools for species identification of Zygomycetes in clinical microbiology laboratories. Fifty-seven strains belonging to 15 species and varieties of Zygomycetes, including Rhizopus , Absidia , Mucor , and Rhizomucor species, were tested for intra- and interspecies variability based on their carbon assimilation profiles. Using ID32C strips, 6 tests were always positive, 7 were never positive, and 18 showed consistently different results between species. With API 50 CH strips, 15 tests were positive for all species, 13 were never positive, and 21 showed different results between species. Nevertheless, assimilation patterns were highly variable among Rhizopus oryzae isolates, and it was not possible to define a specific carbon assimilation profile. With both ID32C and API CH 50 strips, intraspecies variation was found to be low, while large differences were found between genera and species. The clustering of isolates based on their carbon assimilation profiles was in accordance with DNA-based phylogeny of Zygomycetes. In conclusion, carbon assimilation profiles allowed precise and accurate identification of most Zygomycetes to the species level.Keywords
This publication has 30 references indexed in Scilit:
- Genetic Identification of the Main Opportunistic Mucorales by PCR-Restriction Fragment Length PolymorphismJournal of Clinical Microbiology, 2006
- Molecular Identification of Zygomycetes from Culture and Experimentally Infected TissuesJournal of Clinical Microbiology, 2006
- Breakthrough Candida krusei Fungemia during Fluconazole Prophylaxis followed by Breakthrough Zygomycosis during Caspofungin Therapy in a Patient with Severe Aplastic Anemia Who Underwent Stem Cell TransplantationJournal of Clinical Microbiology, 2005
- Novel Perspectives on Mucormycosis: Pathophysiology, Presentation, and ManagementClinical Microbiology Reviews, 2005
- Zygomycosis in a Tertiary‐Care Cancer Center in the Era ofAspergillus‐Active Antifungal Therapy: A Case‐Control Observational Study of 27 Recent CasesThe Journal of Infectious Diseases, 2005
- Breakthrough Fungal Infections in Stem Cell Transplant Recipients Receiving VoriconazoleClinical Infectious Diseases, 2004
- Breakthrough Zygomycosis after Voriconazole Treatment in Recipients of Hematopoietic Stem-Cell TransplantsThe New England Journal of Medicine, 2004
- Experience with the MicroSeq D2 Large-Subunit Ribosomal DNA Sequencing Kit for Identification of Filamentous Fungi Encountered in the Clinical LaboratoryJournal of Clinical Microbiology, 2004
- Epidemiology and Outcome of Mould Infections in Hematopoietic Stem Cell Transplant RecipientsClinical Infectious Diseases, 2002
- Value of substrate utilization data for characterization of Mucor isolatesCanadian Journal of Microbiology, 1996