Carbonic Anhydrase II and Alveolar Fluid Reabsorption during Hypercapnia
- 1 January 2008
- journal article
- Published by American Thoracic Society in American Journal of Respiratory Cell and Molecular Biology
- Vol. 38 (1), 32-37
- https://doi.org/10.1165/rcmb.2007-0121oc
Abstract
Carbonic anhydrase II (CAII) plays an important role in carbon dioxide metabolism and intracellular pH regulation. In this study, we provide evidence that CAII is expressed in both type I (AECI) and type II (AECII) alveolar epithelial cells by RT-PCR and Western blotting in freshly isolated rat cells. These results were further confirmed by double immunostaining with CAII antibodies and AECI- or AECII-specific markers in freshly isolated alveolar epithelial cells and rat lung tissues. Inhibition of CAII by acetazolamide or methazolamide delayed the decrease in the intracellular pH observed during hypercapnia in cultured AECI, AECII, and AECI-like cells. In an isolated-perfused rat lung model, alveolar fluid reabsorption significantly decreased during high CO(2) exposure, which was not prevented by carbonic anhydrase inhibition. Thus, we provide evidence that CAII is expressed in rat alveolar epithelial cells and does not regulate lung alveolar fluid reabsorption during hypercapnia.Keywords
This publication has 36 references indexed in Scilit:
- Inhibition of hypoxia-induced calcium responses in pulmonary arterial smooth muscle by acetazolamide is independent of carbonic anhydrase inhibitionAmerican Journal of Physiology-Lung Cellular and Molecular Physiology, 2007
- Pulmonary vasodilation by acetazolamide during hypoxia is unrelated to carbonic anhydrase inhibitionAmerican Journal of Physiology-Lung Cellular and Molecular Physiology, 2007
- β-Adrenergic Receptor Stimulation and Adenoviral Overexpression of Superoxide Dismutase Prevent the Hypoxia-mediated Decrease in Na,K-ATPase and Alveolar Fluid ReabsorptionPublished by Elsevier BV ,2006
- Mechanisms of pulmonary edema clearanceAmerican Journal of Physiology-Lung Cellular and Molecular Physiology, 2005
- Isolation of highly pure alveolar epithelial type I and type II cells from rat lungsLaboratory Investigation, 2004
- Upregulation of Alveolar Epithelial Active Na + Transport Is Dependent on β 2 -Adrenergic Receptor SignalingCirculation Research, 2004
- Alveolar Type 1 Cells Express the α2 Na,K-ATPase, Which Contributes to Lung Liquid ClearanceCirculation Research, 2003
- The Acute Respiratory Distress SyndromeNew England Journal of Medicine, 2000
- SODIUM CHANNELS IN ALVEOLAR EPITHELIAL CELLS: Molecular Characterization, Biophysical Properties, and Physiological SignificanceAnnual Review of Physiology, 1999
- Direct evidence of participation of rat lung carbonic anhydrase in CO2 reactions.JCI Insight, 1978