Tocotrienol ameliorates behavioral and biochemical alterations in the rat model of alcoholic neuropathy
- 1 September 2009
- journal article
- Published by Ovid Technologies (Wolters Kluwer Health) in Pain
- Vol. 145 (1), 129-135
- https://doi.org/10.1016/j.pain.2009.05.028
Abstract
Chronic alcohol consumption produces a painful peripheral neuropathy for which there is no reliable successful therapy, which is mainly due to lack of understanding of its pathobiology. Alcoholic neuropathy is characterized by spontaneous burning pain, hyperalgesia (an exaggerated pain in response to painful stimuli) and allodynia (a pain evoked by normally innocuous stimuli). Chronic alcohol intake is known to decrease the nociceptive threshold with increased oxidative–nitrosative stress and release of proinflammatory cytokines coupled with activation of protein kinase C. The aim of the present study is to investigate the effect of both isoforms of vitamin E, α-tocopherol (100 mg/kg; oral gavage) and tocotrienol (50, 100 and 200 mg/kg; oral gavage) against alcohol-induced neuropathic pain in rats. Male Wistar rats, were administered 35% v/v ethanol (10 g/kg; oral gavage) for 10 weeks, and were treated with α-tocopherol and tocotrienol for the same duration. Ethanol-treated animals showed a significant decrease in nociceptive threshold as evident from decreased tail flick latency (thermal hyperalgesia) and decreased paw-withdrawal threshold in Randall–Sellito test (mechanical hyperalgesia) and von-Frey hair test (mechanical allodynia) along with the reduction in nerve glutathione and superoxide dismutase levels. TNF-α and IL-1β levels were also significantly increased in both serum and sciatic nerve of ethanol-treated rats. Treatment with α-tocopherol and tocotrienol for 10 weeks significantly improved all the above-stated functional and biochemical deficits in a dose-dependent manner with more potent effects observed with tocotrienol. The study demonstrates the effectiveness of tocotrienol in attenuation of alcoholic neuropathy.Keywords
This publication has 44 references indexed in Scilit:
- Different peripheral mechanisms mediate enhanced nociception in metabolic/toxic and traumatic painful peripheral neuropathies in the ratNeuroscience, 2002
- NMDA receptor regulation by Src kinase signalling in excitatory synaptic transmission and plasticityCurrent Opinion in Neurobiology, 2001
- Key Role for the Epsilon Isoform of Protein Kinase C in Painful Alcoholic Neuropathy in the RatJournal of Neuroscience, 2000
- Serial Determination of Tumor Necrosis Factor-Alpha Content in Rat Sciatic Nerve after Chronic Constriction InjuryExperimental Neurology, 1999
- Ultrastructural Study of the Alterations in Spinal Ganglion Cells of Rats Chronically Fed on EthanolUltrastructural Pathology, 1998
- Quantitative assessment of tactile allodynia in the rat pawJournal of Neuroscience Methods, 1994
- Streptozocin-induced diabetic rats: behavioural evidence for a model of chronic painPain, 1993
- Increased NADH-dependent production of reactive oxygen intermediates by microsomes after chronic ethanol consumption: Comparisons with NADPHArchives of Biochemistry and Biophysics, 1992
- Vitamin E as an in Vitro and in Vivo AntioxidantaAnnals of the New York Academy of Sciences, 1989
- Analysis of nitrate, nitrite, and [15N]nitrate in biological fluidsAnalytical Biochemistry, 1982