Identification of Less-Common Streptococcus pneumoniae Serotypes by a Multiplex PCR-Based Reverse Line Blot Hybridization Assay
- 1 October 2007
- journal article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 45 (10), 3411-3415
- https://doi.org/10.1128/jcm.01076-07
Abstract
We developed a multiplex PCR-based reverse line blot (mPCR/RLB) assay to identify 50 uncommon pneumococcal serotypes. In combination with a previously described mPCR/RLB assay (3), all 90 pneumococcal serotypes can be identified individually (32 serotypes) or, because of predictable cross-reactions, to within small groups of two to five related serotypes (58 serotypes), which can be distinguished using serotype-specific antisera.Keywords
This publication has 11 references indexed in Scilit:
- Discovery of a New Capsular Serotype (6C) within Serogroup 6 ofStreptococcus pneumoniaeJournal of Clinical Microbiology, 2007
- Genotypic identification of presumptive Streptococcus pneumoniae by PCR using four genes highly specific for S. pneumoniaeJournal of Medical Microbiology, 2006
- Multiplex PCR-Based Reverse Line Blot Hybridization Assay To Identify 23 Streptococcus pneumoniae Polysaccharide Vaccine SerotypesJournal of Clinical Microbiology, 2006
- Use of a Serotype-Specific DNA Microarray for Identification of Group B Streptococcus ( Streptococcus agalactiae )Journal of Clinical Microbiology, 2006
- Genetic Analysis of the Capsular Biosynthetic Locus from All 90 Pneumococcal SerotypesPLoS Genetics, 2006
- Real-time assays with molecular beacons and other fluorescent nucleic acid hybridization probesClinica Chimica Acta; International Journal of Clinical Chemistry, 2006
- Immobilization-based isolation of capsule-negative mutants of Streptococcus pneumoniaeMicrobiology, 2005
- A molecular-capsular-type prediction system for 90 Streptococcus pneumoniae serotypes using partial cpsA-cpsB sequencing and wzy- or wzx-specific PCRJournal of Medical Microbiology, 2005
- Using cpsA–cpsB sequence polymorphisms and serotype-/group-specific PCR to predict 51 Streptococcus pneumoniae capsular serotypesJournal of Medical Microbiology, 2003
- Serotype Identification of Group B Streptococci by PCR and SequencingJournal of Clinical Microbiology, 2002