Albumin thiol oxidation and serum protein carbonyl formation are progressively enhanced with advancing stages of chronic kidney disease
- 1 August 2009
- journal article
- Published by Springer Science and Business Media LLC in Clinical and Experimental Nephrology
- Vol. 13 (4), 308-315
- https://doi.org/10.1007/s10157-009-0161-y
Abstract
Oxidative stress is enhanced in advanced chronic kidney disease (CKD) patients and recognized as a main contributor to cardiovascular disease. Carbonyl stress is also known to be enhanced in advanced CKD; however the precise relationship between oxidative stress and carbonyl stress is not clear. The aim of this study was to investigate potential relationships between oxidative stress, carbonyl stress, and renal function among predialysis patients with CKD. A total of 32 predialysis CKD patients (22 male, 10 female) were divided into four groups according to their values for creatinine clearance (Ccr) (group A, ≥60 ml/min; group B, 45–59 ml/min; group C, 30–44 ml/min; group D, ≤29 ml/min). As main markers of oxidative and carbonyl stresses, the redox state of Cys-34 (free thiol group) of human serum albumin [HSA(Cys-34)-redox] and the carbonyl content of serum proteins were employed, respectively. The values for the fraction of both reversibly oxidized HSA [f(HNA-1)] and irreversibly oxidized HSA [f(HNA-2)] significantly increased with a decrease in renal function (group A, 21.0 ± 3.4 and 1.8 ± 0.3%; group D, 31.1 ± 4.1 and 2.7 ± 0.9%, respectively). The value for carbonyl content also significantly increased with a decrease in renal function (group A, 0.7 ± 0.1 nmol/mg protein; group D, 1.1 ± 0.2 nmol/mg protein). There was a significant positive correlation between carbonyl content and the f(HNA-2) value, while such a correlation was not observed between carbonyl content and the f(HNA-1) value, suggesting that there is a close relationship between serum protein carbonylation and irreversible albumin thiol oxidation. There is a close relationship between oxidative stress and carbonyl stress and these are enhanced in correlation with the level of renal dysfunction among predialysis CKD patients.Keywords
This publication has 42 references indexed in Scilit:
- Oral branched‐chain amino acid supplementation improves the oxidized/reduced albumin ratio in patients with liver cirrhosisHepatology Research, 2007
- Dialyzable Uremic Solutes Contribute to Enhanced Oxidation of Serum Albumin in Regular Hemodialysis PatientsBlood Purification, 2007
- Alteration of Redox State of Human Serum Albumin before and after HemodialysisBlood Purification, 2004
- Intra-articular oxidative state correlated with the pathogenesis of disorders of the temporomandibular jointBritish Journal of Oral and Maxillofacial Surgery, 2004
- Chronic Kidney Disease and the Risks of Death, Cardiovascular Events, and HospitalizationNew England Journal of Medicine, 2004
- Increased prevalence of oxidant stress and inflammation in patients with moderate to severe chronic kidney diseaseKidney International, 2004
- Clinical Study: Oxidative Stress in Chronic Renal FailureRenal Failure, 1999
- Age-related change in redox state of human serum albuminBiochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1995
- Antioxidant Enzymes Activity in Polymorphonuclear Leukocytes in Chronic Renal FailureNephron, 1995
- Prediction of Creatinine Clearance from Serum CreatinineNephron, 1976